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==Crimean Congo Hemorrhagic Fever Virus OTU domain in complex with ubiquitin-propargyl.== | ==Crimean Congo Hemorrhagic Fever Virus OTU domain in complex with ubiquitin-propargyl.== | ||
<StructureSection load='3znh' size='340' side='right' caption='[[3znh]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='3znh' size='340' side='right'caption='[[3znh]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3znh]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3znh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crimean-Congo_hemorrhagic_fever_virus_strain_IbAr10200 Crimean-Congo hemorrhagic fever virus strain IbAr10200] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZNH FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AYE:PROP-2-EN-1-AMINE'>AYE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3znh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3znh OCA], [https://pdbe.org/3znh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3znh RCSB], [https://www.ebi.ac.uk/pdbsum/3znh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3znh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/L_CCHFI L_CCHFI] Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3znh" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Thioesterase|Thioesterase]] | *[[Thioesterase 3D structures|Thioesterase 3D structures]] | ||
*[[ | *[[3D structures of ubiquitin|3D structures of ubiquitin]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Crimean-Congo hemorrhagic fever virus strain IbAr10200]] | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Berlin | [[Category: Large Structures]] | ||
[[Category: Ekkebus | [[Category: Berlin I]] | ||
[[Category: Goerdayal | [[Category: Ekkebus R]] | ||
[[Category: Heck | [[Category: Goerdayal G]] | ||
[[Category: Komander | [[Category: Heck AJR]] | ||
[[Category: Kulathu | [[Category: Komander D]] | ||
[[Category: Neefjes | [[Category: Kulathu Y]] | ||
[[Category: Ovaa | [[Category: Neefjes J]] | ||
[[Category: Scholten | [[Category: Ovaa H]] | ||
[[Category: DeJong | [[Category: Scholten A]] | ||
[[Category: VanKasteren | [[Category: DeJong A]] | ||
[[Category: VanKasteren SI]] | |||
Latest revision as of 14:06, 20 December 2023
Crimean Congo Hemorrhagic Fever Virus OTU domain in complex with ubiquitin-propargyl.Crimean Congo Hemorrhagic Fever Virus OTU domain in complex with ubiquitin-propargyl.
Structural highlights
FunctionL_CCHFI Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent innate immunity. Publication Abstract from PubMedActive-site directed probes are powerful in studies of enzymatic function. We report an active-site directed probe based on a warhead so far considered unreactive. By replacing the C-terminal carboxylate of ubiquitin (Ub) with an alkyne functionality, a selective reaction with the active-site cysteine residue of de-ubiquitinating enzymes was observed. The resulting product was shown to be a quaternary vinyl thioether, as determined by X-ray crystallography. Proteomic analysis of proteins bound to an immobilized Ub alkyne probe confirmed the selectivity toward de-ubiquitinating enzymes. The observed reactivity is not just restricted to propargylated Ub, as highlighted by the selective reaction between caspase-1 (interleukin converting enzyme) and a propargylated peptide derived from IL-1beta, a caspase-1 substrate. On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases.,Ekkebus R, van Kasteren SI, Kulathu Y, Scholten A, Berlin I, Geurink PP, de Jong A, Goerdayal S, Neefjes J, Heck AJ, Komander D, Ovaa H J Am Chem Soc. 2013 Feb 15. PMID:23387960[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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