3zkv: Difference between revisions
New page: '''Unreleased structure''' The entry 3zkv is ON HOLD Authors: Gruenwald, M., Lazzaretti, D., Bono, F. Description: Importin13 cytosolic state |
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==Importin13 cytosolic state== | |||
<StructureSection load='3zkv' size='340' side='right'caption='[[3zkv]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3zkv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZKV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zkv OCA], [https://pdbe.org/3zkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zkv RCSB], [https://www.ebi.ac.uk/pdbsum/3zkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zkv ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9VEC5_DROME Q9VEC5_DROME] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Importin13 (Imp13) is a bidirectional karyopherin that can mediate both import and export of cargoes. Imp13 recognizes several import cargoes, which include the exon junction complex components Mago-Y14 and the E2 SUMO-conjugating enzyme Ubc9, and one known export cargo, the translation initiation factor 1A (eIF1A). To understand how Imp13 can perform double duty, we determined the 3.6-A crystal structure of Imp13 in complex with RanGTP and with eIF1A. eIF1A binds at the inner surface of the Imp13 C-terminal arch adjacent and concomitantly to RanGTP illustrating how eIF1A can be exported by Imp13. Moreover, the 3.0-A structure of Imp13 in its unbound state reveals the existence of an open conformation in the cytoplasm that explains export cargo release and completes the export branch of the Imp13 pathway. Finally, we demonstrate that Imp13 is able to bind and export eIF1A in vivo and that its function is essential. | |||
Structural basis for the nuclear export activity of Importin13.,Grunwald M, Lazzaretti D, Bono F EMBO J. 2013 Mar 20;32(6):899-913. doi: 10.1038/emboj.2013.29. Epub 2013 Feb 22. PMID:23435562<ref>PMID:23435562</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3zkv" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Drosophila melanogaster]] | |||
[[Category: Large Structures]] | |||
[[Category: Bono F]] | |||
[[Category: Gruenwald M]] | |||
[[Category: Lazzaretti D]] | |||
Latest revision as of 14:04, 20 December 2023
Importin13 cytosolic stateImportin13 cytosolic state
Structural highlights
FunctionPublication Abstract from PubMedImportin13 (Imp13) is a bidirectional karyopherin that can mediate both import and export of cargoes. Imp13 recognizes several import cargoes, which include the exon junction complex components Mago-Y14 and the E2 SUMO-conjugating enzyme Ubc9, and one known export cargo, the translation initiation factor 1A (eIF1A). To understand how Imp13 can perform double duty, we determined the 3.6-A crystal structure of Imp13 in complex with RanGTP and with eIF1A. eIF1A binds at the inner surface of the Imp13 C-terminal arch adjacent and concomitantly to RanGTP illustrating how eIF1A can be exported by Imp13. Moreover, the 3.0-A structure of Imp13 in its unbound state reveals the existence of an open conformation in the cytoplasm that explains export cargo release and completes the export branch of the Imp13 pathway. Finally, we demonstrate that Imp13 is able to bind and export eIF1A in vivo and that its function is essential. Structural basis for the nuclear export activity of Importin13.,Grunwald M, Lazzaretti D, Bono F EMBO J. 2013 Mar 20;32(6):899-913. doi: 10.1038/emboj.2013.29. Epub 2013 Feb 22. PMID:23435562[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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