3zed: Difference between revisions
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==X-ray structure of the birnavirus VP1-VP3 complex== | ==X-ray structure of the birnavirus VP1-VP3 complex== | ||
<StructureSection load='3zed' size='340' side='right' caption='[[3zed]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='3zed' size='340' side='right'caption='[[3zed]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3zed]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3zed]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Infectious_pancreatic_necrosis_virus Infectious pancreatic necrosis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZED FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zed FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zed OCA], [https://pdbe.org/3zed PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zed RCSB], [https://www.ebi.ac.uk/pdbsum/3zed PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zed ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/RDRP_IPNVJ RDRP_IPNVJ] RNA-dependent RNA polymerase which is found both free and covalently attached to the genomic RNA. May also contain guanylyl and methyl transferase activities (By similarity). | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3zed" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[RNA polymerase|RNA polymerase]] | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Infectious pancreatic necrosis virus]] | [[Category: Infectious pancreatic necrosis virus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Bahar | [[Category: Bahar MW]] | ||
[[Category: Bamford | [[Category: Bamford DH]] | ||
[[Category: Graham | [[Category: Graham SC]] | ||
[[Category: Grimes | [[Category: Grimes JM]] | ||
[[Category: Pang | [[Category: Pang J]] | ||
[[Category: Sarin | [[Category: Sarin LP]] | ||
[[Category: Stuart | [[Category: Stuart DI]] | ||
Latest revision as of 14:00, 20 December 2023
Structural highlights
FunctionRDRP_IPNVJ RNA-dependent RNA polymerase which is found both free and covalently attached to the genomic RNA. May also contain guanylyl and methyl transferase activities (By similarity). Publication Abstract from PubMedInfectious pancreatic necrosis virus (IPNV) - a member of the family Birnaviridae- infects young salmon, with a severe impact on the commercial sea farming industry. Of the five mature proteins encoded by the IPNV genome, the multifunctional VP3 has an essential role in morphogenesis; interacting with the capsid protein VP2, the viral double-stranded RNA (dsRNA) genome and the RNA-dependent RNA-polymerase VP1. Here we investigate one of these VP3 functions and present the crystal structure of the C-terminal 12 residues of VP3 bound to the VP1 polymerase. This interaction, visualised for the first time, reveals the precise molecular determinants used by VP3 to bind the polymerase. Competition binding studies confirm that this region of VP3 is necessary and sufficient for VP1 binding, whilst biochemical experiments show that VP3 attachment has no effect on polymerase activity. These results indicate how VP3 recruits the polymerase into birnavirus capsids during morphogenesis. Structure of a VP1-VP3 complex suggests how birnaviruses package the VP1 polymerase.,Bahar MW, Sarin LP, Graham SC, Pang J, Bamford DH, Stuart DI, Grimes JM J Virol. 2013 Jan 2. PMID:23283942[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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