2y5y: Difference between revisions

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-[[Image:2y5y.png|left|200px]]
-{{STRUCTURE_2y5y|  PDB=2y5y  |  SCENE=  }}
+==Crystal structure of LacY in complex with an affinity inactivator==
+<StructureSection load='2y5y' size='340' side='right'caption='[[2y5y]], [[Resolution|resolution]] 3.38&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2y5y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y5Y FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.38&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=TGA:METHANETHIOSULFONYL-GALACTOSIDE'>TGA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y5y OCA], [https://pdbe.org/2y5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y5y RCSB], [https://www.ebi.ac.uk/pdbsum/2y5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y5y ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Lactose permease of Escherichia coli (LacY) with a single-Cys residue in place of A122 (helix IV) transports galactopyranosides and is specifically inactivated by methanethiosulfonyl-galactopyranosides (MTS-gal), which behave as unique suicide substrates. In order to study the mechanism of inactivation more precisely, we solved the structure of single-Cys122 LacY in complex with covalently bound MTS-gal. This structure exhibits an inward-facing conformation similar to that observed previously with a slight narrowing of the cytoplasmic cavity. MTS-gal is bound covalently, forming a disulfide bond with C122 and positioned between R144 and W151. E269, a residue essential for binding, coordinates the C-4 hydroxyl of the galactopyranoside moiety. The location of the sugar is in accord with many biochemical studies.
-===CRYSTAL STRUCTURE OF LACY IN COMPLEX WITH AN AFFINITY INACTIVATOR===
+Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition.,Chaptal V, Kwon S, Sawaya MR, Guan L, Kaback HR, Abramson J Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9361-6. Epub 2011 May 18. PMID:21593407<ref>PMID:21593407</ref>
-{{ABSTRACT_PUBMED_21593407}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2y5y" style="background-color:#fffaf0;"></div>
-[[2y5y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y5Y OCA].
 ==See Also==
 *[[Lactose Permease|Lactose Permease]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021593407</ref><ref group="xtra">PMID:012471022</ref><references group="xtra"/>
+__TOC__
-[[Category: Escherichia coli]]
+</StructureSection>
-[[Category: Abramson, J.]]
+[[Category: Escherichia coli K-12]]
-[[Category: Chaptal, V.]]
+[[Category: Large Structures]]
-[[Category: Guan, L.]]
+[[Category: Abramson J]]
-[[Category: Kaback, H R.]]
+[[Category: Chaptal V]]
-[[Category: Kwon, S.]]
+[[Category: Guan L]]
-[[Category: Sawaya, M R.]]
+[[Category: Kaback HR]]
-[[Category: Affinity inactivation]]
+[[Category: Kwon S]]
-[[Category: Transport protein]]
+[[Category: Sawaya MR]]