2xu7: Difference between revisions

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-[[Image:2xu7.png|left|200px]]
-{{STRUCTURE_2xu7|  PDB=2xu7  |  SCENE=  }}
+==Structural basis for RbAp48 binding to FOG-1==
+<StructureSection load='2xu7' size='340' side='right'caption='[[2xu7]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2xu7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XU7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XU7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xu7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xu7 OCA], [https://pdbe.org/2xu7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xu7 RCSB], [https://www.ebi.ac.uk/pdbsum/2xu7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xu7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RBBP4_HUMAN RBBP4_HUMAN] Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.<ref>PMID:10866654</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Chromatin modifying complexes such as the NuRD complex are recruited to particular genomic sites by gene-specific nuclear factors. Overall, however, little is known about the molecular basis for these interactions. Here we present the 1.9-Angstrom resolution crystal structure of the NuRD subunit RbAp48 bound to the 15 N-terminal amino acids of the GATA-1 cofactor FOG-1. The FOG-1 peptide contacts a negatively charged binding pocket on top of the RbAp48 beta-propeller that is distinct from the binding surface used by RpAp48 to contact histone H4. We further show that RbAp48 interacts with the NuRD subunit Metastasis Associated-1 (MTA-1) via a surface that is distinct from its FOG-binding pocket, providing a first glimpse into the way in which NuRD assembly facilitates interactions with cofactors. Our RbAp48-FOG structure provides insight into the molecular determinants of FOG-1 dependent association with the NuRD complex and into the links between transcriptional regulation and nucleosome remodelling.
-===STRUCTURAL BASIS FOR RBAP48 BINDING TO FOG-1===
+Insights into the association of the nucleosome remodelling and deacetylase (NURD) complex with friend of gata-1 (FOG-1) from the crystal structure of a retinoblastoma associated protein-48 (RBAP48) fog-1 complex.,Lejon S, Thong SY, Murthy A, Alqarni S, Murzina NV, Blobel GA, Laue ED, Mackay JP J Biol Chem. 2010 Nov 2. PMID:21047798<ref>PMID:21047798</ref>
-{{ABSTRACT_PUBMED_21047798}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2xu7" style="background-color:#fffaf0;"></div>
-[[2xu7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XU7 OCA].
 ==See Also==
-*[[Retinoblastoma-binding protein|Retinoblastoma-binding protein]]
+*[[Retinoblastoma-binding protein 3D structures|Retinoblastoma-binding protein 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021047798</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Blobel, G A.]]
+[[Category: Large Structures]]
-[[Category: Laue, E D.]]
+[[Category: Blobel GA]]
-[[Category: Lejon, S.]]
+[[Category: Laue ED]]
-[[Category: Mackay, J P.]]
+[[Category: Lejon S]]
-[[Category: Murthy, A.]]
+[[Category: Mackay JP]]
-[[Category: Murzina, N V.]]
+[[Category: Murthy A]]
-[[Category: Thong, S Y.]]
+[[Category: Murzina NV]]
-[[Category: Chromatin remodelling]]
+[[Category: Thong SY]]
-[[Category: Corepressor]]
-[[Category: Gata1-mediated repression]]
-[[Category: Histone chaperone]]
-[[Category: Nurd complex]]
-[[Category: Transcription]]