2xga: Difference between revisions

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-{{Seed}}
-[[Image:2xga.jpg|left|200px]]
-<!--
+==MTSL spin-labelled Shigella Flexneri Spa15==
-The line below this paragraph, containing "STRUCTURE_2xga", creates the "Structure Box" on the page.
+<StructureSection load='2xga' size='340' side='right'caption='[[2xga]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2xga]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XGA FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene></td></tr>
-{{STRUCTURE_2xga|  PDB=2xga  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xga OCA], [https://pdbe.org/2xga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xga RCSB], [https://www.ebi.ac.uk/pdbsum/2xga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xga ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPAK_SHIFL SPAK_SHIFL] Required for surface presentation of invasion plasmid antigens. Chaperone specialized in the storage of effectors within the bacterial cytoplasm, maintaining them in a secretion-competent state, and allowing their immediate delivery to target cells upon contact of the bacterium with the host cells. Has been shown to chaperone IpaA, IpgB1, OspC3 and probably also OspB.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Shigella flexneri Spa15 is a chaperone of the type 3 secretion system, which binds a number of effectors to ensure their stabilization prior to secretion. One of these effectors is IpgB1, a mimic of the human Ras-like Rho guanosine triphosphatase RhoG. In this study, Spa15 alone and in complex with IpgB1 has been studied by double electron electron resonance, an experiment that gives distance information showing the spacial separation of attached spin labels. This distance is explained by determining the crystal structure of the spin-labeled Spa15 where labels are seen to be buried in hydrophobic pockets. The double electron electron resonance experiment on the Spa15 complex with IpgB1 shows that IpgB1 does not bind Spa15 in the same way as is seen in the homologous Salmonella sp. chaperone:effector complex InvB:SipA.
-===MTSL SPIN-LABELLED SHIGELLA FLEXNERI SPA15===
+Shigella flexneri Spa15 Crystal Structure Verified in Solution by Double Electron Electron Resonance.,Lillington JE, Lovett JE, Johnson S, Roversi P, Timmel CR, Lea SM J Mol Biol. 2010 Nov 12. PMID:21075116<ref>PMID:21075116</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_21075116}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2xga" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 21075116 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21075116}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-XGA is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGA OCA].
-==Reference==
-<ref group="xtra">PMID:21075116</ref><references group="xtra"/>
 [[Category: Shigella flexneri]]
-[[Category: Johnson, S.]]
+[[Category: Johnson S]]
-[[Category: Lea, S M.]]
+[[Category: Lea SM]]
-[[Category: Lillington, J E.D.]]
+[[Category: Lillington JED]]
-[[Category: Chaperone]]
-[[Category: Virulence factor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec  1 11:27:34 2010''