2x75: Difference between revisions

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-[[Image:2x75.png|left|200px]]
-<!--
+==Staphylococcus aureus adenylosuccinate lyase==
-The line below this paragraph, containing "STRUCTURE_2x75", creates the "Structure Box" on the page.
+<StructureSection load='2x75' size='340' side='right'caption='[[2x75]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2x75]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X75 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
-{{STRUCTURE_2x75|  PDB=2x75  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x75 OCA], [https://pdbe.org/2x75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x75 RCSB], [https://www.ebi.ac.uk/pdbsum/2x75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x75 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PUR8_STAAW PUR8_STAAW] Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.[UniProtKB:P0AB89]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/2x75_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x75 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The medium-resolution structure of adenylosuccinate lyase (PurB) from the bacterial pathogen Staphylococcus aureus in complex with AMP is presented. Oxalate, which is likely to be an artifact of crystallization, has been modelled in the active site and occupies a position close to that where succinate is observed in orthologous structures. PurB catalyzes reactions that support the provision of purines and the control of AMP/fumarate levels. As such, the enzyme is predicted to be essential for the survival of S. aureus and to be a potential therapeutic target. Comparisons of this pathogen PurB with the enzyme from Escherichia coli are presented to allow discussion concerning the enzyme mechanism. Comparisons with human PurB suggest that the close similarity of the active sites would make it difficult to identify species-specific inhibitors for this enzyme. However, there are differences in the way that the subunits are assembled into dimers. The distinct subunit-subunit interfaces may provide a potential area to target by exploiting the observation that creation of the enzyme active site is dependent on oligomerization.
-===Staphylococcus aureus adenylosuccinate lyase===
+Structure of Staphylococcus aureus adenylosuccinate lyase (PurB) and assessment of its potential as a target for structure-based inhibitor discovery.,Fyfe PK, Dawson A, Hutchison MT, Cameron S, Hunter WN Acta Crystallogr D Biol Crystallogr. 2010 Aug;66(Pt 8):881-8. Epub 2010, Jul 9. PMID:20693687<ref>PMID:20693687</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2x75" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20693687}}, adds the Publication Abstract to the page
+*[[Adenylosuccinate lyase|Adenylosuccinate lyase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20693687 is the PubMed ID number.
+*[[Adenylosuccinate lyase 3D structures|Adenylosuccinate lyase 3D structures]]
--->
+== References ==
-{{ABSTRACT_PUBMED_20693687}}
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-[[2x75]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X75 OCA].
+[[Category: Large Structures]]
-==Reference==
-<ref group="xtra">PMID:020693687</ref><references group="xtra"/>
-[[Category: Adenylosuccinate lyase]]
 [[Category: Staphylococcus aureus]]
-[[Category: Cameron, S.]]
+[[Category: Cameron S]]
-[[Category: Dawson, A.]]
+[[Category: Dawson A]]
-[[Category: Fyfe, P K.]]
+[[Category: Fyfe PK]]
-[[Category: Hunter, W N.]]
+[[Category: Hunter WN]]
-[[Category: Hutchison, M T.]]
+[[Category: Hutchison MT]]
-[[Category: Lyase]]
-[[Category: Purine biosynthesis]]
-[[Category: Purine cycle]]