2x6b: Difference between revisions

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-{{Seed}}
-[[Image:2x6b.jpg|left|200px]]
-<!--
+==Potassium Channel from Magnetospirillum Magnetotacticum==
-The line below this paragraph, containing "STRUCTURE_2x6b", creates the "Structure Box" on the page.
+<StructureSection load='2x6b' size='340' side='right'caption='[[2x6b]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2x6b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Magnetospirillum_magnetotacticum Magnetospirillum magnetotacticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X6B FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene></td></tr>
-{{STRUCTURE_2x6b|  PDB=2x6b  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x6b OCA], [https://pdbe.org/2x6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x6b RCSB], [https://www.ebi.ac.uk/pdbsum/2x6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x6b ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IRK10_MAGMG IRK10_MAGMG] Inward rectifier potassium channel that mediates potassium uptake into the cell. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. The inward rectification may be achieved by the blockage of outward current by cytoplasmic divalent metal ions and polyamines. Complements an E.coli mutant that is defective in K(+) uptake.<ref>PMID:20876570</ref> <ref>PMID:20564790</ref> <ref>PMID:22231399</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x6/2x6b_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x6b ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Potassium channels embedded in cell membranes employ gates to regulate K+ current. While a specific constriction in the permeation pathway has historically been implicated in gating, recent reports suggest that the signature ion selectivity filter located in the outer membrane leaflet may be equally important. Inwardly rectifying K+ channels also control the directionality of flow, using intracellular polyamines to stem ion efflux by a valve-like action. This study presents crystallographic evidence of interdependent gates in the conduction pathway and reveals the mechanism of polyamine block. Reorientation of the intracellular domains, concomitant with activation, instigates polyamine release from intracellular binding sites to block the permeation pathway. Conformational adjustments of the slide helices, achieved by rotation of the cytoplasmic assembly relative to the pore, are directly correlated to the ion configuration in the selectivity filter. Ion redistribution occurs irrespective of the constriction, suggesting a more expansive role of the selectivity filter in gating than previously appreciated.
-===POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM===
+Domain reorientation and rotation of an intracellular assembly regulate conduction in Kir potassium channels.,Clarke OB, Caputo AT, Hill AP, Vandenberg JI, Smith BJ, Gulbis JM Cell. 2010 Jun 11;141(6):1018-29. PMID:20564790<ref>PMID:20564790</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2x6b" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-X6B is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Magnetospirillum_magnetotacticum Magnetospirillum magnetotacticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X6B OCA].
+*[[Potassium channel 3D structures|Potassium channel 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Magnetospirillum magnetotacticum]]
-[[Category: Caputo, A T.]]
+[[Category: Caputo AT]]
-[[Category: Clarke, O B.]]
+[[Category: Clarke OB]]
-[[Category: Gulbis, J M.]]
+[[Category: Gulbis JM]]
-[[Category: Hill, A P.]]
+[[Category: Hill AP]]
-[[Category: Smith, B J.]]
+[[Category: Smith BJ]]
-[[Category: Vandenberg, J I.]]
+[[Category: Vandenberg JI]]
-[[Category: Integral membrane protein]]
-[[Category: Ion channel]]
-[[Category: Metal transport]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 23 08:47:11 2010''