2wxt: Difference between revisions

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-[[Image:2wxt.png|left|200px]]
-<!--
+==Clostridium perfringens alpha-toxin strain NCTC8237==
-The line below this paragraph, containing "STRUCTURE_2wxt", creates the "Structure Box" on the page.
+<StructureSection load='2wxt' size='340' side='right'caption='[[2wxt]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2wxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WXT FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_2wxt|  PDB=2wxt  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wxt OCA], [https://pdbe.org/2wxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wxt RCSB], [https://www.ebi.ac.uk/pdbsum/2wxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wxt ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PHLC_CLOP1 PHLC_CLOP1] Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. Acts on human and mouse erythrocytes, but not on rabbit or horse erythrocytes.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wx/2wxt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wxt ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The alpha-toxin produced by Clostridium perfringens is one of the best-studied examples of a toxic phospholipase C. In this study, a nontoxic mutant protein from C. perfringens strain NCTC8237 in which Thr74 is substituted by isoleucine (T74I) has been characterized and is compared with the toxic wild-type protein. Thr74 is part of an exposed loop at the proposed membrane-interfacing surface of the toxin. The mutant protein had markedly reduced cytotoxic and myotoxic activities. However, this substitution did not significantly affect the catalytic activity towards water-soluble substrate or the overall three-dimensional structure of the protein. The data support the proposed role of the 70-90 loop in the recognition of membrane phospholipids. These findings also provide key evidence in support of the hypothesis that the hydrolysis of both phosphatidylcholine and sphingomyelin are required for the cytolytic and toxic activity of phospholipases.
-===CLOSTRIDIUM PERFRINGENS ALPHA-TOXIN STRAIN NCTC8237===
+Comparison of a nontoxic variant of Clostridium perfringens alpha-toxin with the toxic wild-type strain.,Vachieri SG, Clark GC, Alape-Giron A, Flores-Diaz M, Justin N, Naylor CE, Titball RW, Basak AK Acta Crystallogr D Biol Crystallogr. 2010 Oct;66(Pt 10):1067-74. Epub 2010, Sep 18. PMID:20944240<ref>PMID:20944240</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_20944240}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2wxt" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 20944240 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_20944240}}
-==About this Structure==
-[[2wxt]] is a 1 chain structure of [[Gas Gangrene Toxin]] with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WXT OCA].
 ==See Also==
-*[[Gas Gangrene Toxin]]
+*[[Hemolysin 3D structures|Hemolysin 3D structures]]
+*[[Phospholipase C|Phospholipase C]]
-==Reference==
+== References ==
-<ref group="xtra">PMID:20944240</ref><ref group="xtra">PMID:9699639</ref><references group="xtra"/>
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Clostridium perfringens]]
-[[Category: Phospholipase C]]
+[[Category: Large Structures]]
-[[Category: Basak, A K.]]
+[[Category: Basak AK]]
-[[Category: Justin, N.]]
+[[Category: Justin N]]
-[[Category: Naylor, C E.]]
+[[Category: Naylor CE]]
-[[Category: C2 domain]]
-[[Category: Cytolysis]]
-[[Category: Gangrene determinant]]
-[[Category: Hemolysis]]
-[[Category: Hydrolase]]
-[[Category: Membrane binding]]
-[[Category: Virulence]]