2jmn: Difference between revisions

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-[[Image:2jmn.png|left|200px]]
-{{STRUCTURE_2jmn|  PDB=2jmn  |  SCENE=  }}
+==NMR structure of human insulin mutant His-B10-Asp, Pro-B28-Lys, Lys-B29-Pro, 20 structures==
+<StructureSection load='2jmn' size='340' side='right'caption='[[2jmn]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2jmn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1lnp 1lnp] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vks 1vks]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JMN FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jmn OCA], [https://pdbe.org/2jmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jmn RCSB], [https://www.ebi.ac.uk/pdbsum/2jmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jmn ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/INS_HUMAN INS_HUMAN] Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:[https://omim.org/entry/176730 176730].<ref>PMID:3470784</ref> <ref>PMID:2196279</ref> <ref>PMID:4019786</ref> <ref>PMID:1601997</ref>   Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:[https://omim.org/entry/125852 125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.<ref>PMID:18192540</ref>   Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:[https://omim.org/entry/606176 606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.<ref>PMID:17855560</ref> <ref>PMID:18162506</ref>   Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:[https://omim.org/entry/613370 613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.<ref>PMID:18192540</ref> <ref>PMID:18162506</ref> <ref>PMID:20226046</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/INS_HUMAN INS_HUMAN] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jmn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jmn ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Functional surfaces of a protein are often mapped by combination of X-ray crystallography and mutagenesis. Such studies of insulin have yielded paradoxical results, suggesting that the native state is inactive and reorganizes on receptor binding. Of particular interest is the N-terminal alpha-helix of the A-chain. Does this segment function as an alpha-helix or reorganize as recently proposed in a prohormone-convertase complex? To correlate structure and function, we describe a mapping strategy based on protein design. The solution structure of an engineered monomer ([AspB10, LysB28, ProB29]-human insulin) is determined at neutral pH as a template for synthesis of a novel A-chain analogue. Designed by analogy to a protein-folding intermediate, the analogue lacks the A6-A11 disulphide bridge; the cysteine residues are replaced by serine. Its solution structure is remarkable for segmental unfolding of the N-terminal A-chain alpha-helix (A1 to A8) in an otherwise native subdomain. The structure demonstrates that the overall orientation of the A and B chains is consistent with reorganization of the A-chain's N-terminal segment. Nevertheless, the analogue's low biological activity suggests that this segment, a site of clinical mutation causing diabetes mellitus, functions as a preformed recognition alpha-helix.
-===NMR structure of human insulin mutant His-B10-Asp, Pro-B28-Lys, Lys-B29-Pro, 20 structures===
+Mapping the functional surface of insulin by design: structure and function of a novel A-chain analogue.,Hua QX, Hu SQ, Frank BH, Jia W, Chu YC, Wang SH, Burke GT, Katsoyannis PG, Weiss MA J Mol Biol. 1996 Nov 29;264(2):390-403. PMID:8951384<ref>PMID:8951384</ref>
-{{ABSTRACT_PUBMED_8951384}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2jmn" style="background-color:#fffaf0;"></div>
-[[2jmn]] is a 2 chain structure of [[Molecular Playground/Insulin]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entries  and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vks 1vks]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMN OCA].
 ==See Also==
-*[[Molecular Playground/Insulin|Molecular Playground/Insulin]]
+*[[Insulin 3D Structures|Insulin 3D Structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:008951384</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Burke, G T.]]
+[[Category: Large Structures]]
-[[Category: Chu, Y C.]]
+[[Category: Burke GT]]
-[[Category: Frank, B H.]]
+[[Category: Chu YC]]
-[[Category: Hu, S Q.]]
+[[Category: Frank BH]]
-[[Category: Hua, Q X.]]
+[[Category: Hu SQ]]
-[[Category: Jia, W H.]]
+[[Category: Hua QX]]
-[[Category: Katsoyannis, P G.]]
+[[Category: Jia WH]]
-[[Category: Wang, S H.]]
+[[Category: Katsoyannis PG]]
-[[Category: Weiss, M A.]]
+[[Category: Wang SH]]
-[[Category: Hormone]]
+[[Category: Weiss MA]]
-[[Category: Hormone-growth factor complex]]
-[[Category: Human insulin]]
-[[Category: Mutant]]