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<StructureSection load='2a7g'  size='400' side='right' scene= caption='Thermolysin complex with acetate, DMS, Zn+2 (grey) and Ca+2 (green) ions, [[2a7g]]'>
<StructureSection load='2a7g'  size='350' side='right' scene= caption='Thermolysin complex with acetate, DMS, Zn+2 (grey) and Ca+2 (green) ions, [[2a7g]]'>
== Function ==
== Function ==
[[Thermolysin]] (TML) is a thermostable metalloproteinase enzyme from ''Bacillus thermoproteolyticus''.  It catalyzes the hydrolysis of peptide bonds  containing hydrophobic residues. See [[Metalloproteases]]  and [[Matrix metalloproteinase]] for discussion.  
[[Thermolysin]] or '''thermostable neutral proteinase''' (TML) is a thermostable metalloproteinase enzyme from ''Bacillus thermoproteolyticus''.  It catalyzes the hydrolysis of peptide bonds  containing hydrophobic residues. See [[Metalloproteases]]  and [[Matrix metalloproteinase]] for discussion.  


== Structural highlights ==
== Structural highlights ==
Thermolysin is a well researched [[metalloproteases|metalloprotease]] containing <scene name='User:Ralf_Stephan/Sandbox_2/Zinc/2'>zinc</scene> (click this!) and the amino acids His-Glu-X-His-His as its catalytic center. <scene name='User:Ralf_Stephan/Sandbox_2/Res_yellow/3'>Glu-166, His-142 and -146 are grouped around the zinc atom</scene>, holding it fast, while <scene name='User:Ralf_Stephan/Sandbox_2/Res/1'>Glu-143 holds the polarized water atom. Additionally, Tyr-157 and His-231</scene> stabilize the substrate protein which will be cleaved into two smaller proteins.<ref>Matthews, BW. (1988): ''Structural basis of the action of thermolysin and related zinc peptidases''. In: ''Acc. Chem. Res.'' '''21'''(9); 333–340; http://dx.doi.org/10.1021/ar00153a003</ref><ref>PMID:11935352</ref>.  
Thermolysin is a well researched [[metalloproteases|metalloprotease]] containing <scene name='User:Ralf_Stephan/Sandbox_2/Zinc/2'>zinc</scene> (click this!) and the amino acids His-Glu-X-His-His as its catalytic center. <scene name='User:Ralf_Stephan/Sandbox_2/Res_yellow/3'>Glu-166, His-142 and -146 are grouped around the zinc atom</scene>, holding it fast, while <scene name='User:Ralf_Stephan/Sandbox_2/Res/1'>Glu-143 holds the polarized water atom. Additionally, Tyr-157 and His-231</scene> stabilize the substrate protein which will be cleaved into two smaller proteins.<ref>Matthews, BW. (1988): ''Structural basis of the action of thermolysin and related zinc peptidases''. In: ''Acc. Chem. Res.'' '''21'''(9); 333–340; http://dx.doi.org/10.1021/ar00153a003</ref><ref>PMID:11935352</ref>.  
</StructureSection>


== 3D Structures of Thermolysin ==  
== 3D Structures of Thermolysin ==  
[[Thermolysin 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
* Thermolysin
 
**[[2whz]], [[3fvp]], [[3dnz]], [[3do0]], [[3do1]], [[3do2]], [[2g4z]], [[2a7g]], [[1gxw]], [[1kei]], [[1l3f]], [[1tlx]], [[2tlx]], [[8tln]], [[3p7p]], [[3p7q]], [[3p7r]], [[3p7s]], [[3p7t]], [[3p7u]], [[3p7v]], [[3p7w]], [[3zi6]], [[5dlh]], [[5tli]], [[6tli]], [[7tli]], [[8tli]], [[5un3]], [[5uu7]], [[5wr2]], [[5wr3]], [[5wr4]], [[5wr5]], [[5wr6]], [[5a3y]], [[5o8n]], [[5uu8]], [[5uu9]], [[5uua]], [[5uub]], [[5uuc]], [[5uud]], [[5uue]], [[5fsj]], [[5fsp]], [[5fss]], [[5fxn]], [[5t9i]], [[5t9k]], [[5t9q]], [[5tac]], [[5tad]], [[5tae]], [[5tai]], [[5taj]], [[5tak]], [[5hqd]] – TML<br />
**[[5dlh]] – TML - electron crystallography<br />
**[[3ls7]], [[4ow3]], [[4tnl]] - TML residues 233-548<br />
**[[1trl]] - TML residues 255-316 – NMR<br />
**[[3fxs]], [[3fbo]], [[3eim]], [[1lna]], [[1lnb]], [[1lnc]], [[1lnd]], [[1lne]], [[1lnf]] - TML residues 233-548+metal ion replacing Ca<br />
 
*Thermolysin TML+transition state analog
 
**[[1tlp]], [[1tmn]], [[2tmn]], [[4tmn]], [[5tmn]] – TML+transition state analog<br />
 
*Thermolysin + amino acid
 
**[[1kl6]] – TML+ alanine<br />
**[[4m65]] – TML + asparagine<br />
**[[3qgo]] – TML + phenylalanine methyl ester<br />
**[[3qh1]] – TML + benzyloxycarboxyl- aspartate<br />
**[[3qh5]] – TML + benzyloxycarboxyl- aspartate + phenylalanine methyl ester
 
*Thermolysin + dipeptide
 
**[[2wi0]], [[3tmn]], [[8tln]] – TML+ dipeptide <br />
**[[3fgd]] - TML residues 233-548+dipeptide<br />
 
*Thermolysin + inhibitor


**[[1fjo]], [[1fj3]], [[1fjq]], [[1fjt]], [[1fju]], [[1fjv]], [[1fjw]], [[4tli]], [[5tli]], [[6tli]], [[7tli]], [[8tli]], [[1tli]], [[2tli]], [[3tli]] – TML soaked in organic solvents<br />
**[[3fv4]], [[3fxp]], [[3flf]], [[3fb0]], [[3fcq]], [[3f28]], [[3f2p]], [[4mtw]], [[4mwp]], [[4mxj]], [[4mzn]], [[4n4e]], [[4n5p]], [[4n66]], [[4oi5]] – TML residues 233-548+inhibitor<br />
**[[3for]], [[1y3g]], [[1zdp]], [[1z9g]], [[1pes]], [[1pe7]], [[1pe8]], [[1os0]], [[1kto]], [[1ks7]], [[1kro]], [[1kr6]], [[1kkk]], [[1kjo]], [[1kjp]], [[1qf0]], [[1qf1]], [[1qf2]], [[1hyt]], [[1thl]], [[6tmn]], [[7tln]], [[4tln]], [[5tln]], [[1pe5]], [[3ms3]], [[3msa]], [[3msf]], [[3msn]], [[3n21]], [[3nn7]], [[3t73]], [[3t74]], [[3t87]], [[3t8c]], [[3t8d]], [[3t8f]], [[3t8g]], [[3t8h]], [[4h57]], [[4d9w]], [[4d91]], [[5dpe]], [[5dpf]], [[5js3]], [[5jss]], [[5jt9]], [[5jvi]], [[5jxn]], [[5lif]], [[5lvd]], [[5lwd]], [[5m5f]], [[5m69]], [[5mnr]], [[5n2t]], [[5n2x]], [[5n2z]], [[5n31]], [[5n34]], [[5n3v]], [[5n3y]], [[5ma7]], [[5l3u]], [[5l41]], [[5l8p]], [[5m9w]], [[6tmn]], [[7tln]], [[5tln]], [[5tmn]] – TML+inhibitor<br />
**[[3ssb]] – TML + Impi-α<br />
**[[3t2h]] – TML + trimethylamine oxide<br />
**[[3t2i]] – TML + sarcosine<br />
**[[3t2j]] – TML + betaine<br />
}}
== References ==
== References ==
<references/>
<references/>
[[Category: Topic Page]]
[[Category: Topic Page]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel
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