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==Crystal structure of an inactivated Ssp SICLOPPS intein with a CAFHPQ extein== | ==Crystal structure of an inactivated Ssp SICLOPPS intein with a CAFHPQ extein== | ||
<StructureSection load='5ol1' size='340' side='right' caption='[[5ol1]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='5ol1' size='340' side='right'caption='[[5ol1]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5ol1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OL1 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5ol1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OL1 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4 | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.751Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ol1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ol1 OCA], [https://pdbe.org/5ol1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ol1 RCSB], [https://www.ebi.ac.uk/pdbsum/5ol1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ol1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/DPO3A_SYNY3 DPO3A_SYNY3] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 17: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 5ol1" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5ol1" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Synechocystis sp. PCC 6803 substr. Kazusa]] | ||
[[Category: | [[Category: Kick LM]] | ||
[[Category: | [[Category: Schneider S]] | ||
Latest revision as of 19:53, 13 December 2023
Crystal structure of an inactivated Ssp SICLOPPS intein with a CAFHPQ exteinCrystal structure of an inactivated Ssp SICLOPPS intein with a CAFHPQ extein
Structural highlights
FunctionPublication Abstract from PubMedInteins carry out protein-splicing reactions, which are utilised in protein chemistry, protein engineering and biotechnological applications. Rearrangement of the order of the domains in split-inteins results in a head-to-tail cyclisation of the target sequence, which can be used to genetically encode and express libraries of cyclic peptides. The efficiency of the splicing reaction depends on the target sequence. Here we used mass spectrometry to assess the cyclic peptide formation of different, hexameric target sequences in vivo by the DnaE split-inteins from Synechocystis sp. and Nostoc punctiforme, revealing a strong impact by the target sequence and intein on the intracellular peptide concentration. Furthermore, we determined the crystal structures of their pre-splicing complexes, which allowed us to identify F-Block Asp17 as crucial for the DnaE-mediated splicing reaction. Mechanistic insights into cyclic peptide generation by DnaE split-inteins through quantitative and structural investigation.,Kick L, Harteis S, Koch MF, Schneider S Chembiochem. 2017 Sep 15. doi: 10.1002/cbic.201700503. PMID:28914478[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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