2wgz: Difference between revisions

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{{Seed}}
[[Image:2wgz.jpg|left|200px]]


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==Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)==
The line below this paragraph, containing "STRUCTURE_2wgz", creates the "Structure Box" on the page.
<StructureSection load='2wgz' size='340' side='right'caption='[[2wgz]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2wgz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WGZ FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=147:1-O-[P-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>147</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
{{STRUCTURE_2wgz|  PDB=2wgz  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgz OCA], [https://pdbe.org/2wgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wgz RCSB], [https://www.ebi.ac.uk/pdbsum/2wgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wgz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wgz ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The specificities of glycosyltransferases make them useful for the synthesis of biologically active oligosaccharides, but also restrict their range of products. In substrate engineering, substrate promiscuity is enhanced by attaching removable interactive groups to weak substrates. Thus, the attachment of betap-nitrophenyl converts galactose from a poor into a good substrate of alpha-1,3-galactosyltransferase. The crystallographic structure of a complex of alpha3GT containing p-nitrophenyl-beta-galactoside shows that the p-nitrophenyl binds similarly to the N-acetylglucosamine of the substrate, N-acetyllactosamine, interacting with the indole of Trp249. p-Nitrophenyl, unlike N-acetylglucosamine, makes no H-bonds but has more non-polar interactions, making it an effective monosaccharide mimetic.


===CRYSTAL STRUCTURE OF ALPHA-1,3 GALACTOSYLTRANSFERASE (ALPHA3GT) IN A COMPLEX WITH P-NITROPHENYL-BETA-GALACTOSIDE (PNP-BETA-GAL)===
Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal).,Jamaluddin H, Tumbale P, Ferns TA, Thiyagarajan N, Brew K, Acharya KR Biochem Biophys Res Commun. 2009 Aug 7;385(4):601-4. Epub 2009 May 30. PMID:19486884<ref>PMID:19486884</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2wgz" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_19486884}}, adds the Publication Abstract to the page
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 19486884 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_19486884}}
__TOC__
 
</StructureSection>
==About this Structure==
2WGZ is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGZ OCA].
 
==Reference==
<ref group="xtra">PMID:19486884</ref><references group="xtra"/>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Acharya, K R.]]
[[Category: Large Structures]]
[[Category: Brew, K.]]
[[Category: Acharya KR]]
[[Category: Ferns, T A.]]
[[Category: Brew K]]
[[Category: Jamaluddin, H.]]
[[Category: Ferns TA]]
[[Category: Thiyagarajan, N.]]
[[Category: Jamaluddin H]]
[[Category: Tumbale, P.]]
[[Category: Thiyagarajan N]]
[[Category: Alpha-1]]
[[Category: Tumbale P]]
[[Category: Enzyme kinetic]]
[[Category: Galactosyltransferase]]
[[Category: Glycoprotein]]
[[Category: Glycosyltransferase]]
[[Category: Golgi apparatus]]
[[Category: Manganese]]
[[Category: Membrane]]
[[Category: Metal-binding]]
[[Category: Signal-anchor]]
[[Category: Substrate binding]]
[[Category: Transferase]]
[[Category: Transmembrane]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 17 10:13:19 2009''

Latest revision as of 18:55, 13 December 2023

Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)

Structural highlights

2wgz is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.12Å
Ligands:, , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GGTA1_BOVIN Transfer of galactose from UDP-galactose to an acceptor molecule (R).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The specificities of glycosyltransferases make them useful for the synthesis of biologically active oligosaccharides, but also restrict their range of products. In substrate engineering, substrate promiscuity is enhanced by attaching removable interactive groups to weak substrates. Thus, the attachment of betap-nitrophenyl converts galactose from a poor into a good substrate of alpha-1,3-galactosyltransferase. The crystallographic structure of a complex of alpha3GT containing p-nitrophenyl-beta-galactoside shows that the p-nitrophenyl binds similarly to the N-acetylglucosamine of the substrate, N-acetyllactosamine, interacting with the indole of Trp249. p-Nitrophenyl, unlike N-acetylglucosamine, makes no H-bonds but has more non-polar interactions, making it an effective monosaccharide mimetic.

Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal).,Jamaluddin H, Tumbale P, Ferns TA, Thiyagarajan N, Brew K, Acharya KR Biochem Biophys Res Commun. 2009 Aug 7;385(4):601-4. Epub 2009 May 30. PMID:19486884[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Jamaluddin H, Tumbale P, Ferns TA, Thiyagarajan N, Brew K, Acharya KR. Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal). Biochem Biophys Res Commun. 2009 Aug 7;385(4):601-4. Epub 2009 May 30. PMID:19486884 doi:10.1016/j.bbrc.2009.05.111

2wgz, resolution 2.12Å

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