2vuf: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:2vuf.png|left|200px]]
-<!--
+==Human serum albumin complexed with fusidic acid==
-The line below this paragraph, containing "STRUCTURE_2vuf", creates the "Structure Box" on the page.
+<StructureSection load='2vuf' size='340' side='right'caption='[[2vuf]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2vuf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VUF FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUA:FUSIDIC+ACID'>FUA</scene></td></tr>
-{{STRUCTURE_2vuf|  PDB=2vuf  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vuf OCA], [https://pdbe.org/2vuf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vuf RCSB], [https://www.ebi.ac.uk/pdbsum/2vuf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vuf ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[https://omim.org/entry/103600 103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/2vuf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vuf ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bilirubin, an insoluble yellow-orange pigment derived from heme catabolism, accumulates to toxic levels in individuals with impaired or immature liver function. The resulting jaundice may be managed with phototherapy to isomerize the biosynthetic 4Z,15Z-bilirubin-IXalpha to more soluble and excretable isomers, such as 4Z,15E-bilirubin. Bilirubin and its configurational isomers are transported to the liver by human serum albumin (HSA) but their precise binding location(s) on the protein have yet to be determined. To investigate the molecular details of their interaction, we co-crystallised bilirubin with HSA. Strikingly, the crystal structure--determined to 2.42 A resolution--revealed the 4Z,15E-bilirubin-IXalpha isomer bound to an L-shaped pocket in sub-domain IB. We also determined the co-crystal structure of HSA complexed with fusidic acid, an antibiotic that competitively displaces bilirubin from the protein, and showed that it binds to the same pocket. These results provide the first crystal structure of a natural bilirubin pigment bound to serum albumin, challenge some of the present conceptions about HSA-bilirubin interactions, and provide a sound structural framework for finally resolving the long-standing question of where 4Z,15Z-bilirubin-IXalpha binds to the protein.
-===HUMAN SERUM ALBUMIN COMPLEXED WITH FUSIDIC ACID===
+Crystallographic analysis of human serum albumin complexed with 4Z,15E-bilirubin-IXalpha.,Zunszain PA, Ghuman J, McDonagh AF, Curry S J Mol Biol. 2008 Aug 29;381(2):394-406. Epub 2008 Jun 12. PMID:18602119<ref>PMID:18602119</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2vuf" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18602119}}, adds the Publication Abstract to the page
+*[[Albumin 3D structures|Albumin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18602119 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18602119}}
+__TOC__
+</StructureSection>
-==About this Structure==
-VUF is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUF OCA].
-==Reference==
-<ref group="xtra">PMID:18602119</ref><references group="xtra"/>
 [[Category: Homo sapiens]]
-[[Category: Curry, S.]]
+[[Category: Large Structures]]
-[[Category: Ghuman, J.]]
+[[Category: Curry S]]
-[[Category: Zunszain, P A.]]
+[[Category: Ghuman J]]
-[[Category: Albumin]]
+[[Category: Zunszain PA]]
-[[Category: Alternative splicing]]
-[[Category: Carrier protein]]
-[[Category: Cleavage on pair of basic residue]]
-[[Category: Copper]]
-[[Category: Disease mutation]]
-[[Category: Drug-binding]]
-[[Category: Fusidic acid]]
-[[Category: Glycation]]
-[[Category: Glycoprotein]]
-[[Category: Lipid-binding]]
-[[Category: Metal-binding]]
-[[Category: Polymorphism]]
-[[Category: Secreted]]
-[[Category: Transport protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 16:57:18 2009''