2vt1: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2vt1.jpg|left|200px]]
-<!--
+==Crystal structure of the cytoplasmic domain of Spa40, the specificity switch for the Shigella flexneri Type III Secretion System==
-The line below this paragraph, containing "STRUCTURE_2vt1", creates the "Structure Box" on the page.
+<StructureSection load='2vt1' size='340' side='right'caption='[[2vt1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2vt1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VT1 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vt1 OCA], [https://pdbe.org/2vt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vt1 RCSB], [https://www.ebi.ac.uk/pdbsum/2vt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vt1 ProSAT]</span></td></tr>
-{{STRUCTURE_2vt1|  PDB=2vt1  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPAS_SHIFL SPAS_SHIFL] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three ipa proteins.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vt/2vt1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vt1 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The pathogenic bacterium Shigella flexneri uses a type III secretion system to inject virulence factors from the bacterial cytosol directly into host cells. The machinery that identifies secretion substrates and controls the export of extracellular components and effector proteins consists of several inner-membrane and cytoplasmic proteins. One of the inner membrane components, Spa40, belongs to a family of proteins proposed to regulate the switching of substrate specificity of the export apparatus. We show that Spa40 is cleaved within the strictly conserved amino acid sequence NPTH and substitution of the proposed autocatalytic residue abolishes cleavage. Here we also report the crystal structure of the cytoplasmic complex Spa40(C) and compare it with the recent structures of the homologues from Escherichia coli and Salmonella typhimurium. These structures reveal the tight association of the cleaved fragments and show that the conserved NPTH sequence lies on a loop which, when cleaved, swings away from the catalytic N257 residue, resulting in different surface features in this region. This structural rearrangement suggests a mechanism by which non-cleaving forms of these proteins interfere with correct substrate switching of the apparatus.
-'''CRYSTAL STRUCTURE OF THE CYTOPLASMIC DOMAIN OF SPA40, THE SPECIFICITY SWITCH FOR THE SHIGELLA FLEXNERI TYPE III SECRETION SYSTEM'''
+Crystal structure of Spa40, the specificity switch for the Shigella flexneri type III secretion system.,Deane JE, Graham SC, Mitchell EP, Flot D, Johnson S, Lea SM Mol Microbiol. 2008 Jul;69(1):267-76. Epub 2008 May 15. PMID:18485071<ref>PMID:18485071</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-VT1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VT1 OCA].
+<div class="pdbe-citations 2vt1" style="background-color:#fffaf0;"></div>
-[[Category: Shigella flexneri]]
+== References ==
-[[Category: Single protein]]
+<references/>
-[[Category: Deane, J E.]]
+__TOC__
-[[Category: Flot, D.]]
+</StructureSection>
-[[Category: Graham, S C.]]
+[[Category: Large Structures]]
-[[Category: Johnson, S.]]
-[[Category: Lea, S M.]]
-[[Category: Mitchell, E P.]]
-[[Category: Flhb]]
-[[Category: Inner membrane]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Plasmid]]
 [[Category: Shigella flexneri]]
-[[Category: Spa40]]
+[[Category: Deane JE]]
-[[Category: Specificity switch]]
+[[Category: Flot D]]
-[[Category: T3ss]]
+[[Category: Graham SC]]
-[[Category: Transmembrane]]
+[[Category: Johnson S]]
-[[Category: Type iii secretion system]]
+[[Category: Lea SM]]
-[[Category: Virulence]]
+[[Category: Mitchell EP]]
-[[Category: Yscu]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 21:44:38 2008''