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==CRYSTAL STRUCTURE OF E. COLI RNASE E APOPROTEIN - CATALYTIC DOMAIN==
 
<StructureSection load='2vmk' size='340' side='right' caption='[[2vmk]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
==Crystal Structure of E. coli RNase E Apoprotein - Catalytic Domain==
<StructureSection load='2vmk' size='340' side='right'caption='[[2vmk]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2vmk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VMK FirstGlance]. <br>
<table><tr><td colspan='2'>[[2vmk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VMK FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bx2|2bx2]], [[2c0b|2c0b]], [[2c4r|2c4r]], [[1slj|1slj]], [[2vrt|2vrt]], [[1sn8|1sn8]], [[2fym|2fym]], [[1smx|1smx]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vmk OCA], [http://pdbe.org/2vmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vmk RCSB], [http://www.ebi.ac.uk/pdbsum/2vmk PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vmk OCA], [https://pdbe.org/2vmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vmk RCSB], [https://www.ebi.ac.uk/pdbsum/2vmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vmk ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/RNE_ECOLI RNE_ECOLI]] Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic process.  
[https://www.uniprot.org/uniprot/RNE_ECOLI RNE_ECOLI] Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic process.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vm/2vmk_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vm/2vmk_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
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==See Also==
==See Also==
*[[Ribonuclease|Ribonuclease]]
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
*[[Temp|Temp]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Escherichia coli]]
[[Category: Callaghan, A J]]
[[Category: Large Structures]]
[[Category: Koslover, D J]]
[[Category: Callaghan AJ]]
[[Category: Luisi, B F]]
[[Category: Koslover DJ]]
[[Category: Marcaida, M J]]
[[Category: Luisi BF]]
[[Category: Martick, M]]
[[Category: Marcaida MJ]]
[[Category: Scott, W G]]
[[Category: Martick M]]
[[Category: Endonuclease]]
[[Category: Scott WG]]
[[Category: Hydrolase]]
[[Category: Nuclease]]
[[Category: Rna processing]]
[[Category: Rna turnover]]
[[Category: Rna-binding]]

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