2v5j: Difference between revisions
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==Apo Class II aldolase HpcH== | ==Apo Class II aldolase HpcH== | ||
<StructureSection load='2v5j' size='340' side='right' caption='[[2v5j]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='2v5j' size='340' side='right'caption='[[2v5j]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2v5j]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2v5j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_C Escherichia coli C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V5J FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5j OCA], [https://pdbe.org/2v5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v5j RCSB], [https://www.ebi.ac.uk/pdbsum/2v5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/HPCH_ECOLX HPCH_ECOLX] Catalyzes the reversible retro-aldol cleavage of 4-hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinate semialdehyde. Is also able to catalyze the aldol cleavage of 4-hydroxy-2-ketopentanoate and 4-hydroxy-2-ketohexanoate. Is not stereospecific since it can cleave both substrate enantiomers. Also exhibits significant oxaloacetate decarboxylase activity in vitro. In the reverse direction, is able to condense a range of aldehyde acceptors (from two to five carbons in length) with pyruvate or 2-oxobutanoate. Unlike with BphI from Burkholderia xenovorans, the aldol addition reaction lacks stereospecificity, producing a racemic mixture.<ref>PMID:15996099</ref> <ref>PMID:20364820</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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==See Also== | ==See Also== | ||
*[[Aldolase|Aldolase]] | *[[Aldolase 3D structures|Aldolase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Escherichia coli | [[Category: Escherichia coli C]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Bugg TDH]] | ||
[[Category: | [[Category: Fulop V]] | ||
[[Category: | [[Category: Rea D]] | ||
[[Category: | [[Category: Roper DI]] | ||