2uus: Difference between revisions

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-[[Image:2uus.png|left|200px]]
-{{STRUCTURE_2uus|  PDB=2uus  |  SCENE=  }}
+==Crystal structure of the rat FGF1-sucrose octasulfate (SOS) complex.==
+<StructureSection load='2uus' size='340' side='right'caption='[[2uus]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2uus]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GU4:2,3,4,6-TETRA-O-SULFONATO-ALPHA-D-GLUCOPYRANOSE'>GU4</scene>, <scene name='pdbligand=PRD_900013:sucrose+octasulfate'>PRD_900013</scene>, <scene name='pdbligand=YYJ:1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose'>YYJ</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uus OCA], [https://pdbe.org/2uus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uus RCSB], [https://www.ebi.ac.uk/pdbsum/2uus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uus ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FGF1_RAT FGF1_RAT] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/2uus_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uus ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Fibroblast growth factors (FGFs) constitute a family of at least 23 structurally related heparin-binding proteins that are involved in regulation of cell growth, survival, differentiation and migration. Sucrose octasulfate (SOS), a chemical analogue of heparin, has been demonstrated to activate FGF signalling pathways. The structure of rat FGF1 crystallized in the presence of SOS has been determined at 2.2 A resolution. SOS-mediated dimerization of FGF1 was observed, which was further supported by gel-filtration experiments. The major contributors to the sulfate-binding sites in rat FGF1 are Lys113, Lys118, Arg122 and Lys128. An arginine at position 116 is a consensus residue in mammalian FGF molecules; however, it is a serine in rat FGF1. This difference may be important for SOS-mediated FGF1 dimerization in rat.
-===CRYSTAL STRUCTURE OF THE RAT FGF1-SUCROSE OCTASULFATE (SOS) COMPLEX.===
+Dimerization effect of sucrose octasulfate on rat FGF1.,Kulahin N, Kiselyov V, Kochoyan A, Kristensen O, Kastrup JS, Berezin V, Bock E, Gajhede M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jun 1;64(Pt, 6):448-52. Epub 2008 May 16. PMID:18540049<ref>PMID:18540049</ref>
-{{ABSTRACT_PUBMED_18540049}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2uus" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[2uus]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUS OCA].
+*[[Fibroblast growth factor|Fibroblast growth factor]]
+*[[Fibroblast growth factor 3D structures|Fibroblast growth factor 3D structures]]
-==Reference==
+== References ==
-<ref group="xtra">PMID:018540049</ref><references group="xtra"/>
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Rattus norvegicus]]
-[[Category: Berezin, V.]]
+[[Category: Berezin V]]
-[[Category: Bock, E.]]
+[[Category: Bock E]]
-[[Category: Gajhede, M.]]
+[[Category: Gajhede M]]
-[[Category: Kiselyov, V.]]
+[[Category: Kiselyov V]]
-[[Category: Kochoyan, A.]]
+[[Category: Kochoyan A]]
-[[Category: Kristensen, O.]]
+[[Category: Kristensen O]]
-[[Category: Kulahin, N.]]
+[[Category: Kulahin N]]
-[[Category: Angiogenesis]]
-[[Category: Developmental protein]]
-[[Category: Differentiation]]
-[[Category: Fibroblast growth factor]]
-[[Category: Growth factor]]
-[[Category: Heparin-binding]]
-[[Category: Mitogen]]
-[[Category: Sucrose octasulfate]]