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==A NEW CATALYTIC MECHANISM OF PERIPLASMIC NITRATE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 FROM CRYSTALLOGRAPHIC AND EPR DATA AND BASED ON DETAILED ANALYSIS OF THE SIXTH LIGAND==
 
<StructureSection load='2jip' size='340' side='right' caption='[[2jip]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
==A New Catalytic Mechanism of Periplasmic Nitrate Reductase from Desulfovibrio desulfuricans ATCC 27774 from Crystallographic and EPR Data and based on detailed analysis of the sixth ligand==
<StructureSection load='2jip' size='340' side='right'caption='[[2jip]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2jip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JIP FirstGlance]. <br>
<table><tr><td colspan='2'>[[2jip]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JIP FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2nap|2nap]], [[2v3v|2v3v]], [[2jiq|2jiq]], [[2jim|2jim]], [[2v45|2v45]], [[2jio|2jio]], [[2jir|2jir]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jip OCA], [https://pdbe.org/2jip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jip RCSB], [https://www.ebi.ac.uk/pdbsum/2jip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jip ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jip OCA], [http://pdbe.org/2jip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jip RCSB], [http://www.ebi.ac.uk/pdbsum/2jip PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/NAPA_DESDA NAPA_DESDA]] Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.  
[https://www.uniprot.org/uniprot/NAPA_DESDA NAPA_DESDA] Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/2jip_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/2jip_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
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</StructureSection>
</StructureSection>
[[Category: Desulfovibrio desulfuricans]]
[[Category: Desulfovibrio desulfuricans]]
[[Category: Nitrate reductase]]
[[Category: Large Structures]]
[[Category: Brondino, C D]]
[[Category: Brondino CD]]
[[Category: Coelho, C]]
[[Category: Coelho C]]
[[Category: Gonzalez, P J]]
[[Category: Gonzalez PJ]]
[[Category: Moura, I]]
[[Category: Moura I]]
[[Category: Moura, J J.G]]
[[Category: Moura JJG]]
[[Category: Mukhopadhyay, A]]
[[Category: Mukhopadhyay A]]
[[Category: Najmudin, S]]
[[Category: Najmudin S]]
[[Category: Romao, C C]]
[[Category: Romao CC]]
[[Category: Romao, M J]]
[[Category: Romao MJ]]
[[Category: Trincao, J]]
[[Category: Trincao J]]
[[Category: 4fe-4s cluster]]
[[Category: Dissimilatory nitrate reductase]]
[[Category: Disulfide bond]]
[[Category: Electron paramagnetic resonance]]
[[Category: Electron transport]]
[[Category: Iron]]
[[Category: Iron- sulfur]]
[[Category: Iron-sulfur]]
[[Category: Metal-binding]]
[[Category: Molybdenum]]
[[Category: Molybdopterin cofactor]]
[[Category: Nitrate assimilation]]
[[Category: Nitrogenous acceptor]]
[[Category: Oxidoreductase]]
[[Category: Periplasmic]]
[[Category: Sulfido ligand]]
[[Category: Transport]]

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