2j51: Difference between revisions

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-[[Image:2j51.gif|left|200px]]
-<!--
+==Crystal structure of Human STE20-like kinase bound to 5-Amino-3-((4-(aminosulfonyl)phenyl)amino) -N-(2,6-difluorophenyl)-1H-1,2,4-triazole- 1-carbothioamide==
-The line below this paragraph, containing "STRUCTURE_2j51", creates the "Structure Box" on the page.
+<StructureSection load='2j51' size='340' side='right'caption='[[2j51]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2j51]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J51 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DKI:5-AMINO-3-{[4-(AMINOSULFONYL)PHENYL]AMINO}-N-(2,6-DIFLUOROPHENYL)-1H-1,2,4-TRIAZOLE-1-CARBOTHIOAMIDE'>DKI</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
-{{STRUCTURE_2j51|  PDB=2j51  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j51 OCA], [https://pdbe.org/2j51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j51 RCSB], [https://www.ebi.ac.uk/pdbsum/2j51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j51 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SLK_HUMAN SLK_HUMAN] Mediates apoptosis and actin stress fiber dissolution.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j5/2j51_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j51 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Protein kinase autophosphorylation of activation segment residues is a common regulatory mechanism in phosphorylation-dependent signalling cascades. However, the molecular mechanisms that guarantee specific and efficient phosphorylation of these sites have not been elucidated. Here, we report on three novel and diverse protein kinase structures that reveal an exchanged activation segment conformation. This dimeric arrangement results in an active kinase conformation in trans, with activation segment phosphorylation sites in close proximity to the active site of the interacting protomer. Analytical ultracentrifugation and chemical cross-linking confirmed the presence of dimers in solution. Consensus substrate sequences for each kinase showed that the identified activation segment autophosphorylation sites are non-consensus substrate sites. Based on the presented structural and functional data, a model for specific activation segment phosphorylation at non-consensus substrate sites is proposed that is likely to be common to other kinases from diverse subfamilies.
-'''CRYSTAL STRUCTURE OF HUMAN STE20-LIKE KINASE BOUND TO 5-AMINO-3-((4-(AMINOSULFONYL)PHENYL)AMINO)-N-(2,6-DIFLUOROPHENYL)-1H-1,2,4-TRIAZOLE-1-CARBOTHIOAMIDE'''
+Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites.,Pike AC, Rellos P, Niesen FH, Turnbull A, Oliver AW, Parker SA, Turk BE, Pearl LH, Knapp S EMBO J. 2008 Feb 20;27(4):704-14. Epub 2008 Jan 31. PMID:18239682<ref>PMID:18239682</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-J51 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J51 OCA].
+<div class="pdbe-citations 2j51" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Non-specific serine/threonine protein kinase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Arrowsmith C]]
-[[Category: Arrowsmith, C.]]
+[[Category: Debreczeni JE]]
-[[Category: Debreczeni, J E.]]
+[[Category: Edwards A]]
-[[Category: Delft, F Von.]]
+[[Category: Fedorov O]]
-[[Category: Edwards, A.]]
+[[Category: Keates T]]
-[[Category: Fedorov, O.]]
+[[Category: Knapp S]]
-[[Category: Keates, T.]]
+[[Category: Papagrigoriou E]]
-[[Category: Knapp, S.]]
+[[Category: Pike ACW]]
-[[Category: Papagrigoriou, E.]]
+[[Category: Rellos P]]
-[[Category: Pike, A C.W.]]
+[[Category: Salah E]]
-[[Category: Rellos, P.]]
+[[Category: Savitsky P]]
-[[Category: Salah, E.]]
+[[Category: Sundstrom M]]
-[[Category: Savitsky, P.]]
+[[Category: Turnbull AP]]
-[[Category: Sundstrom, M.]]
+[[Category: Weigelt J]]
-[[Category: Turnbull, A P.]]
+[[Category: Von Delft F]]
-[[Category: Weigelt, J.]]
-[[Category: Alternative splicing]]
-[[Category: Apoptosis]]
-[[Category: Atp-binding]]
-[[Category: Coiled coil]]
-[[Category: Germinal centre kinase]]
-[[Category: Kinase]]
-[[Category: Muscle development]]
-[[Category: Nucleotide-binding]]
-[[Category: Phosphorylation]]
-[[Category: Serine/threonine kinase 2]]
-[[Category: Serine/threonine-protein kinase]]
-[[Category: Transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:20:44 2008''