2j4r: Difference between revisions

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-[[Image:2j4r.png|left|200px]]
-{{STRUCTURE_2j4r|  PDB=2j4r  |  SCENE=  }}
+==Structural Study of the Aquifex aeolicus PPX-GPPA enzyme==
+<StructureSection load='2j4r' size='340' side='right'caption='[[2j4r]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2j4r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J4R FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.71&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j4r OCA], [https://pdbe.org/2j4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j4r RCSB], [https://www.ebi.ac.uk/pdbsum/2j4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j4r ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/O67040_AQUAE O67040_AQUAE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j4/2j4r_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j4r ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structure of the prototype exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family member from Aquifex aeolicus in complex with the intracellular second messenger guanosine tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is identified as E119. The dual specificity established for the Escherichia coli homolog is shown to be compatible with a common active site for guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and different degrees of closure between the two domains of the enzyme are associated with substrate binding. The arginines R22 and R267, residing in different domains, are crucial for guanosine pentaphosphate specificity as they interact with the unique 3'-ribose phosphorylation.
-===STRUCTURAL STUDY OF THE AQUIFEX AEOLICUS PPX-GPPA ENZYME===
+Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp.,Kristensen O, Ross B, Gajhede M J Mol Biol. 2008 Feb 1;375(5):1469-76. Epub 2007 Dec 4. PMID:18155044<ref>PMID:18155044</ref>
-{{ABSTRACT_PUBMED_18155044}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2j4r" style="background-color:#fffaf0;"></div>
-[[2j4r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4R OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:018155044</ref><ref group="xtra">PMID:015248747</ref><references group="xtra"/>
+</StructureSection>
-[[Category: Aquifex aeolicus]]
-[[Category: Kristensen, O.]]
 [[Category: Aquifex aeolicus]]
-[[Category: Exopolyphosphatase]]
+[[Category: Large Structures]]
-[[Category: Guanosine pentaphosphate]]
+[[Category: Kristensen O]]
-[[Category: Hydrolase]]
-[[Category: Ppgpp]]
-[[Category: Stringent response]]