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[[Image:2j4d.jpg|left|200px]]<br /><applet load="2j4d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2j4d, resolution 1.9&Aring;" />
'''CRYPTOCHROME 3 FROM ARABIDOPSIS THALIANA'''<br />


==Overview==
==Cryptochrome 3 from Arabidopsis thaliana==
Cryptochromes are almost ubiquitous blue-light receptors and act in, several species as central components of the circadian clock. Despite, being evolutionary and structurally related with DNA photolyases, a class, of light-driven DNA-repair enzymes, and having similar cofactor, compositions, cryptochromes lack DNA-repair activity. Cryptochrome 3 from, the plant Arabidopsis thaliana belongs to the DASH-type subfamily. Its, crystal structure determined at 1.9 Angstroms resolution shows, cryptochrome 3 in a dimeric state with the antenna cofactor, 5,10-methenyltetrahydrofolate (MTHF) bound in a distance of 15.2 Angstroms, to the U-shaped FAD chromophore. Spectroscopic studies on a mutant where a, residue crucial for MTHF-binding, E149, was replaced by site-directed, mutagenesis demonstrate that MTHF acts in cryptochrome 3 as a functional, antenna for the photoreduction of FAD.
<StructureSection load='2j4d' size='340' side='right'caption='[[2j4d]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2j4d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J4D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MHF:5,10-METHENYL-6,7,8-TRIHYDROFOLIC+ACID'>MHF</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j4d OCA], [https://pdbe.org/2j4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j4d RCSB], [https://www.ebi.ac.uk/pdbsum/2j4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j4d ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CRYD_ARATH CRYD_ARATH] May have a photoreceptor function. Binds ss- and ds-DNA in a sequence non-specific manner. Has a photolyase activity specific for cyclobutane pyrimidine dimers in ssDNA.<ref>PMID:17062752</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j4/2j4d_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j4d ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cryptochromes are almost ubiquitous blue-light receptors and act in several species as central components of the circadian clock. Despite being evolutionary and structurally related with DNA photolyases, a class of light-driven DNA-repair enzymes, and having similar cofactor compositions, cryptochromes lack DNA-repair activity. Cryptochrome 3 from the plant Arabidopsis thaliana belongs to the DASH-type subfamily. Its crystal structure determined at 1.9 Angstroms resolution shows cryptochrome 3 in a dimeric state with the antenna cofactor 5,10-methenyltetrahydrofolate (MTHF) bound in a distance of 15.2 Angstroms to the U-shaped FAD chromophore. Spectroscopic studies on a mutant where a residue crucial for MTHF-binding, E149, was replaced by site-directed mutagenesis demonstrate that MTHF acts in cryptochrome 3 as a functional antenna for the photoreduction of FAD.


==About this Structure==
Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna.,Klar T, Pokorny R, Moldt J, Batschauer A, Essen LO J Mol Biol. 2007 Feb 23;366(3):954-64. Epub 2006 Dec 2. PMID:17188299<ref>PMID:17188299</ref>
2J4D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=MHF:'>MHF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mhf+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J4D OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna., Klar T, Pokorny R, Moldt J, Batschauer A, Essen LO, J Mol Biol. 2007 Feb 23;366(3):954-64. Epub 2006 Dec 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17188299 17188299]
</div>
<div class="pdbe-citations 2j4d" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Cryptochrome 3D structures|Cryptochrome 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Batschauer, A.]]
[[Category: Batschauer A]]
[[Category: Essen, L.O.]]
[[Category: Essen L-O]]
[[Category: Klar, T.]]
[[Category: Klar T]]
[[Category: Pokorny, R.]]
[[Category: Pokorny R]]
[[Category: FAD]]
[[Category: MHF]]
[[Category: blue-light response]]
[[Category: chloroplast]]
[[Category: chromophore]]
[[Category: cryptochrome]]
[[Category: dna-binding protein]]
[[Category: fad]]
[[Category: flavoprotein]]
[[Category: mitochondrion]]
[[Category: plastid]]
[[Category: transit peptide]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:41:25 2008''

Latest revision as of 17:33, 13 December 2023

Cryptochrome 3 from Arabidopsis thalianaCryptochrome 3 from Arabidopsis thaliana

Structural highlights

2j4d is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CRYD_ARATH May have a photoreceptor function. Binds ss- and ds-DNA in a sequence non-specific manner. Has a photolyase activity specific for cyclobutane pyrimidine dimers in ssDNA.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cryptochromes are almost ubiquitous blue-light receptors and act in several species as central components of the circadian clock. Despite being evolutionary and structurally related with DNA photolyases, a class of light-driven DNA-repair enzymes, and having similar cofactor compositions, cryptochromes lack DNA-repair activity. Cryptochrome 3 from the plant Arabidopsis thaliana belongs to the DASH-type subfamily. Its crystal structure determined at 1.9 Angstroms resolution shows cryptochrome 3 in a dimeric state with the antenna cofactor 5,10-methenyltetrahydrofolate (MTHF) bound in a distance of 15.2 Angstroms to the U-shaped FAD chromophore. Spectroscopic studies on a mutant where a residue crucial for MTHF-binding, E149, was replaced by site-directed mutagenesis demonstrate that MTHF acts in cryptochrome 3 as a functional antenna for the photoreduction of FAD.

Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna.,Klar T, Pokorny R, Moldt J, Batschauer A, Essen LO J Mol Biol. 2007 Feb 23;366(3):954-64. Epub 2006 Dec 2. PMID:17188299[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Selby CP, Sancar A. A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity. Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17696-700. Epub 2006 Oct 24. PMID:17062752 doi:http://dx.doi.org/0607993103
  2. ↑ Klar T, Pokorny R, Moldt J, Batschauer A, Essen LO. Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna. J Mol Biol. 2007 Feb 23;366(3):954-64. Epub 2006 Dec 2. PMID:17188299 doi:10.1016/j.jmb.2006.11.066

2j4d, resolution 1.90Å

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