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[[Image:2j0v.gif|left|200px]]<br />
<applet load="2j0v" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2j0v, resolution 1.78&Aring;" />
'''THE CRYSTAL STRUCTURE OF ARABIDOPSIS THALIANA RAC7-ROP9: THE FIRST RAS SUPERFAMILY GTPASE FROM THE PLANT KINGDOM'''<br />


==Overview==
==The crystal structure of Arabidopsis thaliana RAC7-ROP9: the first RAS superfamily GTPase from the plant kingdom==
Arabidopsis thaliana RAC/ROP GTPases constitute a plant specific Rho, GTPase family in the RAS superfamily, which has been implicated in, numerous pivotal signalling cascades in plants. Research has shown that, plants in some cases have evolved different modes of regulating Rho GTPase, activity as compared to the equivalent systems in animals and yeast. In, order to gain structural insight into plant signaling at the molecular, level, we have determined the first crystal structure of a RAC-like GTPase, belonging to the RAS superfamily from the plant kingdom. The structure of, AtRAC7/ROP9 bound to GDP was solved at a resolution of 1.78 A. We have, found that the structure of plant Rho GTPases is based upon a conserved, G-domain architecture, but structural differences were found concerning, the insert region and switch II region of the protein.
<StructureSection load='2j0v' size='340' side='right'caption='[[2j0v]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2j0v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J0V FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j0v OCA], [https://pdbe.org/2j0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j0v RCSB], [https://www.ebi.ac.uk/pdbsum/2j0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j0v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RAC7_ARATH RAC7_ARATH] Acts as a negative regulator of abscisic acid (ABA) responses.<ref>PMID:12417701</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/2j0v_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j0v ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Arabidopsis thaliana RAC/ROP GTPases constitute a plant specific Rho GTPase family in the RAS superfamily, which has been implicated in numerous pivotal signalling cascades in plants. Research has shown that plants in some cases have evolved different modes of regulating Rho GTPase activity as compared to the equivalent systems in animals and yeast. In order to gain structural insight into plant signaling at the molecular level, we have determined the first crystal structure of a RAC-like GTPase belonging to the RAS superfamily from the plant kingdom. The structure of AtRAC7/ROP9 bound to GDP was solved at a resolution of 1.78 A. We have found that the structure of plant Rho GTPases is based upon a conserved G-domain architecture, but structural differences were found concerning the insert region and switch II region of the protein.


==About this Structure==
The crystal structure of Arabidopsis thaliana RAC7/ROP9: the first RAS superfamily GTPase from the plant kingdom.,Sormo CG, Leiros I, Brembu T, Winge P, Os V, Bones AM Phytochemistry. 2006 Nov;67(21):2332-40. Epub 2006 Sep 26. PMID:17005216<ref>PMID:17005216</ref>
2J0V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J0V OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The crystal structure of Arabidopsis thaliana RAC7/ROP9: the first RAS superfamily GTPase from the plant kingdom., Sormo CG, Leiros I, Brembu T, Winge P, Os V, Bones AM, Phytochemistry. 2006 Nov;67(21):2332-40. Epub 2006 Sep 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17005216 17005216]
</div>
<div class="pdbe-citations 2j0v" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bones, A.M.]]
[[Category: Bones AM]]
[[Category: Brembu, T.]]
[[Category: Brembu T]]
[[Category: Leiros, I.]]
[[Category: Leiros I]]
[[Category: Os, V.]]
[[Category: Os V]]
[[Category: Sormo, C.G.]]
[[Category: Sormo CG]]
[[Category: Winge, P.]]
[[Category: Winge P]]
[[Category: GDP]]
[[Category: MG]]
[[Category: abscisic acid signaling pathway]]
[[Category: arabidopsis thaliana]]
[[Category: atrac7]]
[[Category: dna binding protein nucleotide-binding]]
[[Category: gtp-binding]]
[[Category: lipoprotein]]
[[Category: membrane]]
[[Category: palmitate]]
[[Category: ras superfamily]]
[[Category: rho gtpase]]
[[Category: rop9]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 18:22:17 2007''

Latest revision as of 17:31, 13 December 2023

The crystal structure of Arabidopsis thaliana RAC7-ROP9: the first RAS superfamily GTPase from the plant kingdomThe crystal structure of Arabidopsis thaliana RAC7-ROP9: the first RAS superfamily GTPase from the plant kingdom

Structural highlights

2j0v is a 4 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.78Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RAC7_ARATH Acts as a negative regulator of abscisic acid (ABA) responses.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Arabidopsis thaliana RAC/ROP GTPases constitute a plant specific Rho GTPase family in the RAS superfamily, which has been implicated in numerous pivotal signalling cascades in plants. Research has shown that plants in some cases have evolved different modes of regulating Rho GTPase activity as compared to the equivalent systems in animals and yeast. In order to gain structural insight into plant signaling at the molecular level, we have determined the first crystal structure of a RAC-like GTPase belonging to the RAS superfamily from the plant kingdom. The structure of AtRAC7/ROP9 bound to GDP was solved at a resolution of 1.78 A. We have found that the structure of plant Rho GTPases is based upon a conserved G-domain architecture, but structural differences were found concerning the insert region and switch II region of the protein.

The crystal structure of Arabidopsis thaliana RAC7/ROP9: the first RAS superfamily GTPase from the plant kingdom.,Sormo CG, Leiros I, Brembu T, Winge P, Os V, Bones AM Phytochemistry. 2006 Nov;67(21):2332-40. Epub 2006 Sep 26. PMID:17005216[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Zheng ZL, Nafisi M, Tam A, Li H, Crowell DN, Chary SN, Schroeder JI, Shen J, Yang Z. Plasma membrane-associated ROP10 small GTPase is a specific negative regulator of abscisic acid responses in Arabidopsis. Plant Cell. 2002 Nov;14(11):2787-97. PMID:12417701 doi:10.1105/tpc.005611
  2. ↑ Sormo CG, Leiros I, Brembu T, Winge P, Os V, Bones AM. The crystal structure of Arabidopsis thaliana RAC7/ROP9: the first RAS superfamily GTPase from the plant kingdom. Phytochemistry. 2006 Nov;67(21):2332-40. Epub 2006 Sep 26. PMID:17005216 doi:10.1016/j.phytochem.2006.08.011

2j0v, resolution 1.78Å

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