2ixb: Difference between revisions

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-{{Seed}}
-[[Image:2ixb.png|left|200px]]
-<!--
+==Crystal structure of N-ACETYLGALACTOSAMINIDASE in complex with GalNAC==
-The line below this paragraph, containing "STRUCTURE_2ixb", creates the "Structure Box" on the page.
+<StructureSection load='2ixb' size='340' side='right'caption='[[2ixb]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2ixb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IXB FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-{{STRUCTURE_2ixb|  PDB=2ixb  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ixb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ixb OCA], [https://pdbe.org/2ixb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ixb RCSB], [https://www.ebi.ac.uk/pdbsum/2ixb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ixb ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GH109_ELIME GH109_ELIME]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/2ixb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ixb ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical practice has been the lack of efficient glycosidase enzymes. Here we report two bacterial glycosidase gene families that provide enzymes capable of efficient removal of A and B antigens at neutral pH with low consumption of recombinant enzymes. The crystal structure of a member of the alpha-N-acetylgalactosaminidase family reveals an unusual catalytic mechanism involving NAD+. The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions.
-===CRYSTAL STRUCTURE OF N-ACETYLGALACTOSAMINIDASE IN COMPLEX WITH GALNAC===
+Bacterial glycosidases for the production of universal red blood cells.,Liu QP, Sulzenbacher G, Yuan H, Bennett EP, Pietz G, Saunders K, Spence J, Nudelman E, Levery SB, White T, Neveu JM, Lane WS, Bourne Y, Olsson ML, Henrissat B, Clausen H Nat Biotechnol. 2007 Apr;25(4):454-64. Epub 2007 Apr 1. PMID:17401360<ref>PMID:17401360</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-IXB is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IXB OCA].
+<div class="pdbe-citations 2ixb" style="background-color:#fffaf0;"></div>
-[[Category: Alpha-N-acetylgalactosaminidase]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Elizabethkingia meningoseptica]]
-[[Category: Bourne, Y.]]
+[[Category: Large Structures]]
-[[Category: Clausen, H.]]
+[[Category: Bourne Y]]
-[[Category: Henrissat, B.]]
+[[Category: Clausen H]]
-[[Category: Liu, Q P.]]
+[[Category: Henrissat B]]
-[[Category: Sulzenbacher, G.]]
+[[Category: Liu QP]]
-[[Category: A-eco conversion]]
+[[Category: Sulzenbacher G]]
-[[Category: Hydrolase]]
-[[Category: N-acetylgalactosaminidase]]
-[[Category: Nad]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:30:38 2009''