2iwm: Difference between revisions

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-[[Image:2iwm.jpg|left|200px]]
- {{Structure
+==precursor mutant Cys1Ser of Penicillin V Acylase from Bacillus sphaericus==
-|PDB= 2iwm |SIZE=350|CAPTION= <scene name='initialview01'>2iwm</scene>, resolution 2.50&Aring;
+<StructureSection load='2iwm' size='340' side='right'caption='[[2iwm]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[2iwm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IWM FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-|GENE=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iwm OCA], [https://pdbe.org/2iwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iwm RCSB], [https://www.ebi.ac.uk/pdbsum/2iwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iwm ProSAT]</span></td></tr>
-}}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PAC_LYSSH PAC_LYSSH] The enzyme catalyzes the conversion of penicillin to 6-aminopenicillanate The precursor, furthermore, acts as a self-processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iw/2iwm_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iwm ConSurf].
+<div style="clear:both"></div>
-'''PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS'''
+==See Also==
+*[[Penicillin acylase|Penicillin acylase]]
+__TOC__
-==Overview==
+</StructureSection>
-The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.
+[[Category: Large Structures]]
-==About this Structure==
-IWM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWM OCA].
-==Reference==
-Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16508111 16508111]
 [[Category: Lysinibacillus sphaericus]]
-[[Category: Penicillin amidase]]
+[[Category: Chandra PM]]
-[[Category: Single protein]]
+[[Category: Dodson G]]
-[[Category: Chandra, P M.]]
+[[Category: Suresh CG]]
-[[Category: Dodson, G.]]
-[[Category: Suresh, C G.]]
-[[Category: antibiotic resistance]]
-[[Category: autoproteolysis]]
-[[Category: hydrolase]]
-[[Category: penicillin]]
-[[Category: precursor]]
-[[Category: zymogen]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:34:17 2008''