2cfp: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2cfp.png|left|200px]]
-{{STRUCTURE_2cfp|  PDB=2cfp  |  SCENE=  }}
+==Sugar Free Lactose Permease at acidic pH==
+<StructureSection load='2cfp' size='340' side='right'caption='[[2cfp]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2cfp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CFP FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfp OCA], [https://pdbe.org/2cfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cfp RCSB], [https://www.ebi.ac.uk/pdbsum/2cfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cfp ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cfp_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cfp ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cation-coupled active transport is an essential cellular process found ubiquitously in all living organisms. Here, we present two novel ligand-free X-ray structures of the lactose permease (LacY) of Escherichia coli determined at acidic and neutral pH, and propose a model for the mechanism of coupling between lactose and H+ translocation. No sugar-binding site is observed in the absence of ligand, and deprotonation of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction.
-===SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH===
+Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY.,Mirza O, Guan L, Verner G, Iwata S, Kaback HR EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9. PMID:16525509<ref>PMID:16525509</ref>
-{{ABSTRACT_PUBMED_16525509}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2cfp" style="background-color:#fffaf0;"></div>
-[[2cfp]] is a 1 chain structure of [[Lactose Permease]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFP OCA].
 ==See Also==
 *[[Lactose Permease|Lactose Permease]]
-*[[Major Facilitators|Major Facilitators]]
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:016525509</ref><references group="xtra"/>
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Guan, L.]]
+[[Category: Large Structures]]
-[[Category: Iwata, S.]]
+[[Category: Guan L]]
-[[Category: Kaback, H R.]]
+[[Category: Iwata S]]
-[[Category: Mirza, O.]]
+[[Category: Kaback HR]]
-[[Category: Verner, G.]]
+[[Category: Mirza O]]
-[[Category: Formylation]]
+[[Category: Verner G]]
-[[Category: Lactose permease]]
-[[Category: Lactose/h+ symport]]
-[[Category: Sugar transport]]
-[[Category: Transmembrane]]
-[[Category: Transport]]
-[[Category: Transport mechanism]]