2c8f: Difference between revisions

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-[[Image:2c8f.jpg|left|200px]]
-<!--
+==Structure of the ARTT motif E214N mutant C3bot1 Exoenzyme (NAD-bound state, crystal form III)==
-The line below this paragraph, containing "STRUCTURE_2c8f", creates the "Structure Box" on the page.
+<StructureSection load='2c8f' size='340' side='right'caption='[[2c8f]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2c8f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C8F FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-{{STRUCTURE_2c8f|  PDB=2c8f  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8f OCA], [https://pdbe.org/2c8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c8f RCSB], [https://www.ebi.ac.uk/pdbsum/2c8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8f ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q7M0L1_CLOBO Q7M0L1_CLOBO]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/2c8f_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c8f ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+C3-like exoenzymes are ADP-ribosyltransferases that specifically modify some Rho GTPase proteins, leading to their sequestration in the cytoplasm, and thus inhibiting their regulatory activity on the actin cytoskeleton. This modification process goes through three sequential steps involving NAD-hydrolysis, Rho recognition, and binding, leading to Rho ADP-ribosylation. Independently, three distinct residues within the ARTT loop of the C3 exoenzymes are critical for each of these steps. Supporting the critical role of the ARTT loop, we have shown previously that it adopts a distinct conformation upon NAD binding. Here, we present seven wild-type and ARTT loop-mutant structures of C3 exoenzyme of Clostridium botulinum free and bound to its true substrate, NAD, and to its NAD-hydrolysis product, nicotinamide. Altogether, these structures expand our understanding of the conformational diversity of the C3 exoenzyme, mainly within the ARTT loop.
-'''STRUCTURE OF THE ARTT MOTIF E214N MUTANT C3BOT1 EXOENZYME (NAD-BOUND STATE, CRYSTAL FORM III)'''
+Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes.,Menetrey J, Flatau G, Boquet P, Menez A, Stura EA Protein Sci. 2008 May;17(5):878-86. Epub 2008 Mar 27. PMID:18369192<ref>PMID:18369192</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2c8f" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-C8F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8F OCA].
+*[[Exoenzyme 3D structures|Exoenzyme 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Clostridium botulinum]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Boquet, P.]]
+[[Category: Boquet P]]
-[[Category: Flatau, G.]]
+[[Category: Flatau G]]
-[[Category: Menetrey, J.]]
+[[Category: Menetrey J]]
-[[Category: Menez, A.]]
+[[Category: Menez A]]
-[[Category: Stura, E A.]]
+[[Category: Stura EA]]
-[[Category: Adp-ribosyltransferase]]
-[[Category: Artt motif]]
-[[Category: Bacterial toxin]]
-[[Category: C3 exoenzyme]]
-[[Category: Glycosyltransferase]]
-[[Category: Transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 21:26:26 2008''