2c45: Difference between revisions

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-[[Image:2c45.png|left|200px]]
-<!--
+==NATIVE PRECURSOR OF PYRUVOYL DEPENDENT ASPARTATE DECARBOXYLASE==
-The line below this paragraph, containing "STRUCTURE_2c45", creates the "Structure Box" on the page.
+<StructureSection load='2c45' size='340' side='right'caption='[[2c45]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2c45]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C45 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.99&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c45 OCA], [https://pdbe.org/2c45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c45 RCSB], [https://www.ebi.ac.uk/pdbsum/2c45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c45 ProSAT]</span></td></tr>
-{{STRUCTURE_2c45|  PDB=2c45  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PAND_MYCTU PAND_MYCTU] Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/2c45_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c45 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+L-aspartate-alpha-decarboxylase (ADC) is a critical regulatory enzyme in the pantothenate biosynthetic pathway and belongs to a small class of self-cleaving and pyruvoyl-dependent amino acid decarboxylases. The expression level of ADC in Mycobacterium tuberculosis (Mtb) was confirmed by cDNA analysis, immunoblotting with an anti-ADC polyclonal antibody using whole cell lysate and immunoelectron microscopy. The recombinant ADC proenzyme from Mycobacterium tuberculosis (MtbADC) was overexpressed in E. coli and the protein structure was determined at 2.99 A resolution. The proteins fold into the double-psi beta-barrel structure. The subunits of the two tetramers (there are eight ADC molecules in the asymmetric unit) form pseudo fourfold rotational symmetry, similar to the E. coli ADC proenzyme structure. As pantothenate is synthesized in microorganisms, plants, and fungi but not in animals, structure elucidation of Mtb ADC is of substantial interest for structure-based drug development.
-===NATIVE PRECURSOR OF PYRUVOYL DEPENDENT ASPARTATE DECARBOXYLASE===
+Crystal structure of uncleaved L-aspartate-alpha-decarboxylase from Mycobacterium tuberculosis.,Gopalan G, Chopra S, Ranganathan A, Swaminathan K Proteins. 2006 Dec 1;65(4):796-802. PMID:17001646<ref>PMID:17001646</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2c45" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_17001646}}, adds the Publication Abstract to the page
+*[[Aspartate decarboxylase 3D structures|Aspartate decarboxylase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 17001646 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17001646}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[2c45]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C45 OCA].
+[[Category: Mycobacterium tuberculosis]]
+[[Category: Chopra S]]
-==Reference==
+[[Category: Gopalan G]]
-<ref group="xtra">PMID:17001646</ref><ref group="xtra">PMID:9546220</ref><ref group="xtra">PMID:14633979</ref><ref group="xtra">PMID:15184017</ref><references group="xtra"/>
+[[Category: Ranganathan A]]
-[[Category: Aspartate 1-decarboxylase]]
+[[Category: Swaminathan K]]
-[[Category: Chopra, S.]]
-[[Category: Gopalan, G.]]
-[[Category: Ranganathan, A.]]
-[[Category: Swaminathan, K.]]
-[[Category: Carboxylase]]
-[[Category: Decarboxylase]]
-[[Category: Double-psi beta barrel]]
-[[Category: Lyase]]
-[[Category: Pantothenate biosynthesis]]
-[[Category: Pyruvate]]
-[[Category: Zymogen]]