2bk0: Difference between revisions

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-[[Image:2bk0.png|left|200px]]
-{{STRUCTURE_2bk0|  PDB=2bk0  |  SCENE=  }}
+==Crystal structure of the major celery allergen Api G 1==
+<StructureSection load='2bk0' size='340' side='right'caption='[[2bk0]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2bk0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Apium_graveolens Apium graveolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BK0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bk0 OCA], [https://pdbe.org/2bk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bk0 RCSB], [https://www.ebi.ac.uk/pdbsum/2bk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bk0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ALL1_APIGR ALL1_APIGR]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/2bk0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bk0 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Many patients who have been sensitised to pollen, display allergic symptoms after ingestion of certain plant food such as fresh fruit, vegetables and nuts. The cause is the cross-reactivity between structurally very similar major plant allergens. In particular, allergy to celery is very frequently associated with birch and mugwort pollen sensitization, known as to the birch-mugwort-celery syndrome. The crystal structure of the major celery allergen Api g 1, a homologue of the major birch pollen allergen Bet v 1, has been determined to a resolution of 2.9 A. The structure of Api g 1 is very similar to that of Bet v 1 with major differences occurring in the segment comprised of residues 23-45, preceding the well conserved glycine-rich P-loop, as well as in loops beta3-beta4 and beta5-beta6. In particular, Api g 1 lacks E45, which has been shown to be a crucial residue for antibody recognition in the crystal complex of Bet v 1 with the Fab fragment of a murine monoclonal IgG (BV16) antibody. The absence of E45 and the structural differences in the preceding segment suggest that this region of the Api g 1 surface is probably not responsible for the observed cross-reactivity with Bet v 1. A detailed analysis of the molecular surface in combination with sequence alignment revealed three conserved surface patches which may account for cross-reactivity with Bet v 1. Several residues of Bet v 1 which have been shown by mutagenesis studies to be involved in IgE recognition belong to these conserved surface regions. The structure of Api g 1 and the related epitope analysis provides a molecular basis for a better understanding of allergen cross-reactivity and may lead to the development of hypoallergens which would allow a safer immunotherapy.
-===CRYSTAL STRUCTURE OF THE MAJOR CELERY ALLERGEN API G 1===
+Crystal structure of the major celery allergen Api g 1: molecular analysis of cross-reactivity.,Schirmer T, Hoffimann-Sommergrube K, Susani M, Breiteneder H, Markovic-Housley Z J Mol Biol. 2005 Sep 2;351(5):1101-9. PMID:16051263<ref>PMID:16051263</ref>
-{{ABSTRACT_PUBMED_16051263}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2bk0" style="background-color:#fffaf0;"></div>
-[[2bk0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Apium_graveolens Apium graveolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BK0 OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:016051263</ref><references group="xtra"/>
+</StructureSection>
 [[Category: Apium graveolens]]
-[[Category: Breiteneder, H.]]
+[[Category: Large Structures]]
-[[Category: Hoffmann-Sommergruber, K.]]
+[[Category: Breiteneder H]]
-[[Category: Markovic-Housley, Z.]]
+[[Category: Hoffmann-Sommergruber K]]
-[[Category: Schirmer, T.]]
+[[Category: Markovic-Housley Z]]
-[[Category: Allergen]]
+[[Category: Schirmer T]]
-[[Category: Bet v 1-related protein]]
-[[Category: Cross reactive epitope]]
-[[Category: Major celery allergen api g 1]]
-[[Category: Pathogenesis-related protein]]
-[[Category: Plant defense]]