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[[Image:2bjf.gif|left|200px]]<br />
<applet load="2bjf" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2bjf, resolution 1.67&Aring;" />
'''CRYSTAL STRUCTURE OF CONJUGATED BILE ACID HYDROLASE FROM CLOSTRIDIUM PERFRINGENS IN COMPLEX WITH REACTION PRODUCTS TAURINE AND DEOXYCHOLATE'''<br />


==Overview==
==Crystal Structure of Conjugated Bile Acid Hydrolase from Clostridium perfringens in Complex with Reaction Products Taurine and Deoxycholate==
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile, acids in the mammalian gut. The crystal structures of conjugated bile acid, hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex, with taurodeoxycholate that was hydrolyzed to the reaction products, taurine and deoxycholate are described here at 2.1 and 1.7 A resolution, respectively. The crystal structures reveal close relationship between, CBAH and penicillin V acylase from Bacillus sphaericus. This similarity, together with the N-terminal cysteine classifies CBAH as a member of the, N-terminal nucleophile (Ntn) hydrolase superfamily. Both crystal, structures show an identical homotetrameric organization with dihedral, (D(2) or 222) point group symmetry. The structure analysis of C., perfringens CBAH identifies critical residues in catalysis, substrate, recognition, and tetramer formation which may serve in further biochemical, characterization of bile acid hydrolases.
<StructureSection load='2bjf' size='340' side='right'caption='[[2bjf]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DXC:(3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC+ACID'>DXC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TAU:2-AMINOETHANESULFONIC+ACID'>TAU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjf OCA], [https://pdbe.org/2bjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bjf RCSB], [https://www.ebi.ac.uk/pdbsum/2bjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CBH_CLOPE CBH_CLOPE] The enzyme catalyzes the degradation of conjugated bile acids in the mammalian gut.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bjf ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction products taurine and deoxycholate are described here at 2.1 and 1.7 A resolution, respectively. The crystal structures reveal close relationship between CBAH and penicillin V acylase from Bacillus sphaericus. This similarity together with the N-terminal cysteine classifies CBAH as a member of the N-terminal nucleophile (Ntn) hydrolase superfamily. Both crystal structures show an identical homotetrameric organization with dihedral (D(2) or 222) point group symmetry. The structure analysis of C. perfringens CBAH identifies critical residues in catalysis, substrate recognition, and tetramer formation which may serve in further biochemical characterization of bile acid hydrolases.


==About this Structure==
Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product.,Rossocha M, Schultz-Heienbrok R, von Moeller H, Coleman JP, Saenger W Biochemistry. 2005 Apr 19;44(15):5739-48. PMID:15823032<ref>PMID:15823032</ref>
2BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens] with DXC, TAU and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Choloylglycine_hydrolase Choloylglycine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.24 3.5.1.24] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BJF OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product., Rossocha M, Schultz-Heienbrok R, von Moeller H, Coleman JP, Saenger W, Biochemistry. 2005 Apr 19;44(15):5739-48. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15823032 15823032]
</div>
[[Category: Choloylglycine hydrolase]]
<div class="pdbe-citations 2bjf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clostridium perfringens]]
[[Category: Clostridium perfringens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Coleman, J.P.]]
[[Category: Coleman JP]]
[[Category: Moeller, H.Von.]]
[[Category: Rossocha M]]
[[Category: Rossocha, M.]]
[[Category: Saenger W]]
[[Category: Saenger, W.]]
[[Category: Schultz-Heienbrok R]]
[[Category: Schultz-Heienbrok, R.]]
[[Category: Von Moeller H]]
[[Category: DXC]]
[[Category: GOL]]
[[Category: TAU]]
[[Category: amidohydrolase]]
[[Category: bile acids]]
[[Category: bsh]]
[[Category: hydrolase]]
[[Category: ntn-hydrolase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:32:10 2007''

Latest revision as of 16:40, 13 December 2023

Crystal Structure of Conjugated Bile Acid Hydrolase from Clostridium perfringens in Complex with Reaction Products Taurine and DeoxycholateCrystal Structure of Conjugated Bile Acid Hydrolase from Clostridium perfringens in Complex with Reaction Products Taurine and Deoxycholate

Structural highlights

2bjf is a 1 chain structure with sequence from Clostridium perfringens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.67Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CBH_CLOPE The enzyme catalyzes the degradation of conjugated bile acids in the mammalian gut.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction products taurine and deoxycholate are described here at 2.1 and 1.7 A resolution, respectively. The crystal structures reveal close relationship between CBAH and penicillin V acylase from Bacillus sphaericus. This similarity together with the N-terminal cysteine classifies CBAH as a member of the N-terminal nucleophile (Ntn) hydrolase superfamily. Both crystal structures show an identical homotetrameric organization with dihedral (D(2) or 222) point group symmetry. The structure analysis of C. perfringens CBAH identifies critical residues in catalysis, substrate recognition, and tetramer formation which may serve in further biochemical characterization of bile acid hydrolases.

Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product.,Rossocha M, Schultz-Heienbrok R, von Moeller H, Coleman JP, Saenger W Biochemistry. 2005 Apr 19;44(15):5739-48. PMID:15823032[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Rossocha M, Schultz-Heienbrok R, von Moeller H, Coleman JP, Saenger W. Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product. Biochemistry. 2005 Apr 19;44(15):5739-48. PMID:15823032 doi:http://dx.doi.org/10.1021/bi0473206

2bjf, resolution 1.67Å

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