2bgq: Difference between revisions

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-[[Image:2bgq.gif|left|200px]]<br /><applet load="2bgq" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2bgq, resolution 2.50&Aring;" />
-'''APO ALDOSE REDUCTASE FROM BARLEY'''<br />
-==Overview==
+==apo aldose reductase from barley==
-In most higher plants a period of desiccation is the terminal event in embryogenesis. Excised barley embryos acquire desiccation tolerance at a precise developmental stage and cDNA clones have been isolated which are temporally linked with desiccation tolerance. One such clone (pG22-69) with a putative gene product of 34 kd displays high structural homology to mammalian genes encoding an NADPH dependent aldose reductase involved in the synthesis of sorbitol. This first aldose reductase gene of plants is expressed constitutively during embryo maturation and is modulated by the plant hormones abscisic acid (ABA) and gibberellic acid (GA). Immunohistochemistry showed that the protein is preferentially expressed in tissues formed at early stages in embryogenesis. Measurements of enzymatic activity indicate that pG22-69 encodes an active aldose reductase. The finding of this reductase activity and the cloning of the corresponding gene supports the existence of a metabolic pathway in plants playing a role in the synthesis of osmolytes like sorbitol. The significance of this work is that genes of related structure and functions are being used in diverse organisms to fulfil stress related biological requirements.
+<StructureSection load='2bgq' size='340' side='right'caption='[[2bgq]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2bgq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BGQ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bgq OCA], [https://pdbe.org/2bgq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bgq RCSB], [https://www.ebi.ac.uk/pdbsum/2bgq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bgq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ALDR_HORVU ALDR_HORVU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bg/2bgq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bgq ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-BGQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Aldehyde_reductase Aldehyde reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.21 1.1.1.21] Known structural/functional Site: <scene name='pdbsite=AC1:Binding+Site+For+Residue+So4+A+1321'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGQ OCA].
+*[[Aldose reductase 3D structures|Aldose reductase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-An ABA and GA modulated gene expressed in the barley embryo encodes an aldose reductase related protein., Bartels D, Engelhardt K, Roncarati R, Schneider K, Rotter M, Salamini F, EMBO J. 1991 May;10(5):1037-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1827067 1827067]
-[[Category: Aldehyde reductase]]
 [[Category: Hordeum vulgare]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Christensen, C L.]]
+[[Category: Christensen CL]]
-[[Category: Henriksen, A.]]
+[[Category: Henriksen A]]
-[[Category: Olsen, J G.]]
+[[Category: Olsen JG]]
-[[Category: Olsen, O.]]
+[[Category: Olsen O]]
-[[Category: Pedersen, L.]]
+[[Category: Pedersen L]]
-[[Category: SO4]]
-[[Category: aldo/keto reductase oxidoreductase]]
-[[Category: aldose reductase]]
-[[Category: apoenzyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Mar 14 09:41:52 2008''