1vyh: Difference between revisions
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==PAF-AH Holoenzyme: Lis1/Alfa2== | ==PAF-AH Holoenzyme: Lis1/Alfa2== | ||
<StructureSection load='1vyh' size='340' side='right' caption='[[1vyh]], [[Resolution|resolution]] 3.40Å' scene=''> | <StructureSection load='1vyh' size='340' side='right'caption='[[1vyh]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1vyh]] is a 20 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1vyh]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VYH FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vyh OCA], [https://pdbe.org/1vyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vyh RCSB], [https://www.ebi.ac.uk/pdbsum/1vyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vyh ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/PA1B2_HUMAN PA1B2_HUMAN] Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 31: | Line 30: | ||
==See Also== | ==See Also== | ||
*[[Phospholipase A2|Phospholipase A2]] | *[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Mus musculus]] | ||
[[Category: Derewenda | [[Category: Derewenda ZS]] | ||
[[Category: Knapp | [[Category: Knapp S]] | ||
[[Category: Massimiliano | [[Category: Massimiliano L]] | ||
[[Category: Monzani | [[Category: Monzani S]] | ||
[[Category: Musacchio | [[Category: Musacchio A]] | ||
[[Category: Perrina | [[Category: Perrina F]] | ||
[[Category: Tarricone | [[Category: Tarricone C]] | ||
[[Category: Tsai | [[Category: Tsai L-H]] | ||
Latest revision as of 16:08, 13 December 2023
PAF-AH Holoenzyme: Lis1/Alfa2PAF-AH Holoenzyme: Lis1/Alfa2
Structural highlights
FunctionPA1B2_HUMAN Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedMutations in the LIS1 gene cause lissencephaly, a human neuronal migration disorder. LIS1 binds dynein and the dynein-associated proteins Nde1 (formerly known as NudE), Ndel1 (formerly known as NUDEL), and CLIP-170, as well as the catalytic alpha dimers of brain cytosolic platelet activating factor acetylhydrolase (PAF-AH). The mechanism coupling the two diverse regulatory pathways remains unknown. We report the structure of LIS1 in complex with the alpha2/alpha2 PAF-AH homodimer. One LIS1 homodimer binds symmetrically to one alpha2/alpha2 homodimer via the highly conserved top faces of the LIS1 beta propellers. The same surface of LIS1 contains sites of mutations causing lissencephaly and overlaps with a putative dynein binding surface. Ndel1 competes with the alpha2/alpha2 homodimer for LIS1, but the interaction is complex and requires both the N- and C-terminal domains of LIS1. Our data suggest that the LIS1 molecule undergoes major conformational rearrangement when switching from a complex with the acetylhydrolase to the one with Ndel1. Coupling PAF signaling to dynein regulation: structure of LIS1 in complex with PAF-acetylhydrolase.,Tarricone C, Perrina F, Monzani S, Massimiliano L, Kim MH, Derewenda ZS, Knapp S, Tsai LH, Musacchio A Neuron. 2004 Dec 2;44(5):809-21. PMID:15572112[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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