1vyh: Difference between revisions

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-[[Image:1vyh.png|left|200px]]
-{{STRUCTURE_1vyh|  PDB=1vyh  |  SCENE=  }}
+==PAF-AH Holoenzyme: Lis1/Alfa2==
+<StructureSection load='1vyh' size='340' side='right'caption='[[1vyh]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1vyh]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VYH FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vyh OCA], [https://pdbe.org/1vyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vyh RCSB], [https://www.ebi.ac.uk/pdbsum/1vyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vyh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PA1B2_HUMAN PA1B2_HUMAN] Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/1vyh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vyh ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Mutations in the LIS1 gene cause lissencephaly, a human neuronal migration disorder. LIS1 binds dynein and the dynein-associated proteins Nde1 (formerly known as NudE), Ndel1 (formerly known as NUDEL), and CLIP-170, as well as the catalytic alpha dimers of brain cytosolic platelet activating factor acetylhydrolase (PAF-AH). The mechanism coupling the two diverse regulatory pathways remains unknown. We report the structure of LIS1 in complex with the alpha2/alpha2 PAF-AH homodimer. One LIS1 homodimer binds symmetrically to one alpha2/alpha2 homodimer via the highly conserved top faces of the LIS1 beta propellers. The same surface of LIS1 contains sites of mutations causing lissencephaly and overlaps with a putative dynein binding surface. Ndel1 competes with the alpha2/alpha2 homodimer for LIS1, but the interaction is complex and requires both the N- and C-terminal domains of LIS1. Our data suggest that the LIS1 molecule undergoes major conformational rearrangement when switching from a complex with the acetylhydrolase to the one with Ndel1.
-===PAF-AH HOLOENZYME: LIS1/ALFA2===
+Coupling PAF signaling to dynein regulation: structure of LIS1 in complex with PAF-acetylhydrolase.,Tarricone C, Perrina F, Monzani S, Massimiliano L, Kim MH, Derewenda ZS, Knapp S, Tsai LH, Musacchio A Neuron. 2004 Dec 2;44(5):809-21. PMID:15572112<ref>PMID:15572112</ref>
-{{ABSTRACT_PUBMED_15572112}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1vyh" style="background-color:#fffaf0;"></div>
-[[1vyh]] is a 20 chain structure of [[Platelet-activating factor acetylhydrolase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VYH OCA].
 ==See Also==
-*[[Platelet-activating factor acetylhydrolase|Platelet-activating factor acetylhydrolase]]
+*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:015572112</ref><references group="xtra"/>
+__TOC__
-[[Category: 1-alkyl-2-acetylglycerophosphocholine esterase]]
+</StructureSection>
 [[Category: Homo sapiens]]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Derewenda, Z S.]]
+[[Category: Derewenda ZS]]
-[[Category: Knapp, S.]]
+[[Category: Knapp S]]
-[[Category: Massimiliano, L.]]
+[[Category: Massimiliano L]]
-[[Category: Monzani, S.]]
+[[Category: Monzani S]]
-[[Category: Musacchio, A.]]
+[[Category: Musacchio A]]
-[[Category: Perrina, F.]]
+[[Category: Perrina F]]
-[[Category: Tarricone, C.]]
+[[Category: Tarricone C]]
-[[Category: Tsai, L H.]]
+[[Category: Tsai L-H]]
-[[Category: Acetylhydrolase]]
-[[Category: Cell division]]
-[[Category: Cytoskeleton]]
-[[Category: Hydrolase]]
-[[Category: Lissencephaly]]
-[[Category: Mitosis]]
-[[Category: Neurogenesis]]
-[[Category: Platelet activacting factor]]
-[[Category: Regulator of cytoplasmic dynein]]