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[[Image:1uw6.gif|left|200px]]<br />
<applet load="1uw6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1uw6, resolution 2.2&Aring;" />
'''X-RAY STRUCTURE OF ACETYLCHOLINE BINDING PROTEIN (ACHBP) IN COMPLEX WITH NICOTINE'''<br />


==Overview==
==X-ray structure of acetylcholine binding protein (AChBP) in complex with nicotine==
Nicotinic acetylcholine receptors are prototypes for the pharmaceutically, important family of pentameric ligand-gated ion channels. Here we present, atomic resolution structures of nicotine and carbamylcholine binding to, AChBP, a water-soluble homolog of the ligand binding domain of nicotinic, receptors and their family members, GABAA, GABAC, 5HT3 serotonin, and, glycine receptors. Ligand binding is driven by enthalpy and is accompanied, by conformational changes in the ligand binding site. Residues in the, binding site contract around the ligand, with the largest movement in the, C loop. As expected, the binding is characterized by substantial aromatic, and hydrophobic contributions, but additionally there are close contacts, between protein oxygens and positively charged groups in the ligands. The, higher affinity of nicotine is due to a main chain hydrogen bond with the, B loop and a closer packing of the aromatic groups. These structures will, be useful tools for the development of new drugs involving nicotinic, acetylcholine receptor-associated diseases.
<StructureSection load='1uw6' size='340' side='right'caption='[[1uw6]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1uw6]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Lymnaea_stagnalis Lymnaea stagnalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UW6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UW6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCT:(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE'>NCT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uw6 OCA], [https://pdbe.org/1uw6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uw6 RCSB], [https://www.ebi.ac.uk/pdbsum/1uw6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uw6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ACHP_LYMST ACHP_LYMST] Binds to acetylcholine. Modulates neuronal synaptic transmission.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uw/1uw6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uw6 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Nicotinic acetylcholine receptors are prototypes for the pharmaceutically important family of pentameric ligand-gated ion channels. Here we present atomic resolution structures of nicotine and carbamylcholine binding to AChBP, a water-soluble homolog of the ligand binding domain of nicotinic receptors and their family members, GABAA, GABAC, 5HT3 serotonin, and glycine receptors. Ligand binding is driven by enthalpy and is accompanied by conformational changes in the ligand binding site. Residues in the binding site contract around the ligand, with the largest movement in the C loop. As expected, the binding is characterized by substantial aromatic and hydrophobic contributions, but additionally there are close contacts between protein oxygens and positively charged groups in the ligands. The higher affinity of nicotine is due to a main chain hydrogen bond with the B loop and a closer packing of the aromatic groups. These structures will be useful tools for the development of new drugs involving nicotinic acetylcholine receptor-associated diseases.


==About this Structure==
Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures.,Celie PH, van Rossum-Fikkert SE, van Dijk WJ, Brejc K, Smit AB, Sixma TK Neuron. 2004 Mar 25;41(6):907-14. PMID:15046723<ref>PMID:15046723</ref>
1UW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lymnaea_stagnalis Lymnaea stagnalis] with NCT as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UW6 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures., Celie PH, van Rossum-Fikkert SE, van Dijk WJ, Brejc K, Smit AB, Sixma TK, Neuron. 2004 Mar 25;41(6):907-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15046723 15046723]
</div>
<div class="pdbe-citations 1uw6" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
*[[Cation-pi interactions|Cation-pi interactions]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lymnaea stagnalis]]
[[Category: Lymnaea stagnalis]]
[[Category: Single protein]]
[[Category: Brejc K]]
[[Category: Brejc, K.]]
[[Category: Celie PHN]]
[[Category: Celie, P.H.N.]]
[[Category: Sixma TK]]
[[Category: Dijk, W.J.Van.]]
[[Category: Smit AB]]
[[Category: Rossum-Fikkert, S.E.Van.]]
[[Category: Van Dijk WJ]]
[[Category: Sixma, T.K.]]
[[Category: Van Rossum-fikkert SE]]
[[Category: Smit, A.B.]]
[[Category: NCT]]
[[Category: acetylcholine]]
[[Category: glycoprotein]]
[[Category: igg fold]]
[[Category: nicotine]]
[[Category: pentamer]]
 
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