1olp: Difference between revisions

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-{{Seed}}
-[[Image:1olp.png|left|200px]]
-<!--
+==Alpha Toxin from Clostridium Absonum==
-The line below this paragraph, containing "STRUCTURE_1olp", creates the "Structure Box" on the page.
+<StructureSection load='1olp' size='340' side='right'caption='[[1olp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1olp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_sardiniense Clostridium sardiniense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLP FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_1olp|  PDB=1olp  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1olp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1olp OCA], [https://pdbe.org/1olp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1olp RCSB], [https://www.ebi.ac.uk/pdbsum/1olp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1olp ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8GCY3_CLOSR Q8GCY3_CLOSR]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/1olp_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1olp ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Clostridium absonum phospholipase C (Caa) is a 42.7 kDa protein, which shows 60% amino acid sequence identity with the Clostridium perfringens phospholipase C, or alpha-toxin (Cpa), and has been isolated from patients suffering from gas gangrene. We report the cloning and sequencing, purification, characterisation and crystal structure of the Caa enzyme. Caa had twice the phospholipid-hydrolysing (lecithinase) activity, 1.5 times the haemolytic activity and over seven times the activity towards phosphatidylcholine-based liposomes when compared with Cpa. However, the Caa enzyme had a lower activity than Cpa to the free (i.e. not in lipid bilayer) substrate para-nitrophenylphosphorylcholine, towards sphingomyelin-based liposomes and showed half the cytotoxicity. The lethal dose (LD(50)) of Caa in mice was approximately twice that of Cpa. The crystal structure of Caa shows that the 72-93 residue loop is in a conformation different from those of previously determined open-form alpha-toxin structures. This conformational change suggests a role for W84 in membrane binding and a possible route of entry into the active site along a hydrophobic channel created by the re-arrangement of this loop. Overall, the properties of Caa are compatible with a role as a virulence-determinant in gas gangrene caused by C.absonum.
-===ALPHA TOXIN FROM CLOSTRIDIUM ABSONUM===
+Clostridium absonum alpha-toxin: new insights into clostridial phospholipase C substrate binding and specificity.,Clark GC, Briggs DC, Karasawa T, Wang X, Cole AR, Maegawa T, Jayasekera PN, Naylor CE, Miller J, Moss DS, Nakamura S, Basak AK, Titball RW J Mol Biol. 2003 Oct 31;333(4):759-69. PMID:14568535<ref>PMID:14568535</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1olp" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_14568535}}, adds the Publication Abstract to the page
+*[[Hemolysin 3D structures|Hemolysin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 14568535 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_14568535}}
+__TOC__
+</StructureSection>
-==About this Structure==
-OLP is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_sardiniense Clostridium sardiniense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLP OCA].
-==Reference==
-<ref group="xtra">PMID:14568535</ref><references group="xtra"/>
 [[Category: Clostridium sardiniense]]
-[[Category: Phospholipase C]]
+[[Category: Large Structures]]
-[[Category: Basak, A K.]]
+[[Category: Basak AK]]
-[[Category: Briggs, D C.]]
+[[Category: Briggs DC]]
-[[Category: Calcium binding]]
-[[Category: Gas gangrene determinant]]
-[[Category: Hydrolase]]
-[[Category: Membrane binding]]
-[[Category: Zinc phospholipase c]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:32:33 2009''