1gv3: Difference between revisions

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-[[Image:1gv3.png|left|200px]]
-<!--
+==The 2.0 Angstrom resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase==
-The line below this paragraph, containing "STRUCTURE_1gv3", creates the "Structure Box" on the page.
+<StructureSection load='1gv3' size='340' side='right'caption='[[1gv3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1gv3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GV3 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-{{STRUCTURE_1gv3|  PDB=1gv3  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gv3 OCA], [https://pdbe.org/1gv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gv3 RCSB], [https://www.ebi.ac.uk/pdbsum/1gv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gv3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8Z0M1_NOSS1 Q8Z0M1_NOSS1]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gv/1gv3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gv3 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cyanobacteria are shown to be unique in containing membrane-bound manganese superoxide dismutases (MnSOD). They are homodimeric type 2 membrane proteins that protect this phototrophic organism against oxidative stress. We have determined, for the first time, the 2.0A resolution structure of the catalytic portion of the MnSOD from the filamentous cyanobacterium Anabaena PCC 7120. Within each subunit, both the N-terminal helical hairpin (His94 and His145) and the C-terminal alpha/beta domain (His232 and Asp228) contribute ligands to the catalytic manganese site. Together with a water or hydroxide ion (OH(x)) a five-coordinated trigonal bipyramidal geometry is formed, with OH(x) and His90 forming the axial ligands and manganese shifted out of the equatorial plane in the direction of OH(x). The ligands including OH(x) are tightly constrained by hydrogen bonding with surrounding residues either from the same monomer (Tyr98, Asn144, Trp194, Gln213, Val229, Trp230) or from the neighbouring subunit (Glu231, Tyr235). This underlines the important role of the symmetric dimeric structure of MnSODs in contributing elements to both the active site and the substrate funnel. The Mn cdots, three dots, centered Mn distance (18.4A) is bridged by the hydrogen-bonded His232 of one monomer with Glu231 of the other monomer. A detailed discussion of the structure, a comparison with known structures of soluble MnSODs as well as a model of the cyanobacterial membrane-bound MnSOD is presented.
-===THE 2.0 ANGSTROM RESOLUTION STRUCTURE OF THE CATALYTIC PORTION OF A CYANOBACTERIAL MEMBRANE-BOUND MANGANESE SUPEROXIDE DISMUTASE===
+The 2.0A resolution structure of the catalytic portion of a cyanobacterial membrane-bound manganese superoxide dismutase.,Atzenhofer W, Regelsberger G, Jacob U, Peschek G, Furtmuller P, Huber R, Obinger C J Mol Biol. 2002 Aug 16;321(3):479-89. PMID:12162960<ref>PMID:12162960</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_12162960}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1gv3" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 12162960 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_12162960}}
-==About this Structure==
-[[1gv3]] is a 2 chain structure of [[Superoxide Dismutase]] with sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GV3 OCA].
 ==See Also==
-*[[Superoxide Dismutase]]
+*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:12162960</ref><references group="xtra"/>
+__TOC__
-[[Category: Anabaena sp.]]
+</StructureSection>
-[[Category: Atzenhofer, W.]]
+[[Category: Large Structures]]
-[[Category: Huber, R.]]
+[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
-[[Category: Jacob, U.]]
+[[Category: Atzenhofer W]]
-[[Category: Obinger, C.]]
+[[Category: Huber R]]
-[[Category: Peschek, G A.]]
+[[Category: Jacob U]]
-[[Category: Regelsberger, G.]]
+[[Category: Obinger C]]
-[[Category: Anabaena pcc 7120]]
+[[Category: Peschek GA]]
-[[Category: Crystal structure]]
+[[Category: Regelsberger G]]
-[[Category: Manganese superoxide dismutase]]