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[[Image:1gt5.gif|left|200px]]


{{Structure
==Complexe of Bovine Odorant Binding Protein with benzophenone==
|PDB= 1gt5 |SIZE=350|CAPTION= <scene name='initialview01'>1gt5</scene>, resolution 2.08&Aring;
<StructureSection load='1gt5' size='340' side='right'caption='[[1gt5]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
|SITE= <scene name='pdbsite=BZA:Bzq+Binding+Site+For+Chain+B'>BZA</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=BZQ:DIPHENYLMETHANONE'>BZQ</scene>
<table><tr><td colspan='2'>[[1gt5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GT5 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.08&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BZQ:DIPHENYLMETHANONE'>BZQ</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gt5 OCA], [https://pdbe.org/1gt5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gt5 RCSB], [https://www.ebi.ac.uk/pdbsum/1gt5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gt5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OBP_BOVIN OBP_BOVIN] This protein binds a wide variety of chemical odorants.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/1gt5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gt5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of bovine odorant-binding protein (bOBP) revealed a striking feature of a dimer formed by domain swapping [Tegoni, M., Ramoni, R., Bignetti, E., Spinelli, S. &amp; Cambillau, C. (1996) Nat. Struct. Biol.3, 863-867; Bianchet, M.A., Bains, G., Pelosi, P., Pevsner, J., Snyder, S.H., Monaco, H.L. &amp; Amzel, L.M. (1996) Nat. Struct. Biol.3, 934-939] and the presence of a naturally occuring ligand [Ramoni, R., Vincent, F., Grolli, S., Conti, V., Malosse, C., Boyer, F.D., Nagnan-Le Meillour, P., Spinelli, S., Cambillau, C. &amp; Tegoni, M. (2001) J. Biol. Chem.276, 7150-7155]. These features led us to investigate the binding of odorant molecules with bOBP in solution and in the crystal. The behavior of odorant molecules in bOBP resembles that observed with porcine OBP (pOBP), although the latter is monomeric and devoid of ligand when purified. The odorant molecules presented K(d) values with bOBP in the micromolar range. Most of the X-ray structures revealed that odorant molecules interact with a common set of residues forming the cavity wall and do not exhibit specific interactions. Depending on the ligand and on the monomer (A or B), a single residue--Phe89--presents alternate conformations and might control cross-talking between the subunits. Crystal data on both pOBP and bOBP, in contrast with binding and spectroscopic studies on rat OBP in solution, reveal an absence of significant conformational changes involving protein loops or backbone. Thus, the role of OBP in signal triggering remains unresolved.


'''COMPLEXE OF BOVINE ODORANT BINDING PROTEIN WITH BENZOPHENONE'''
Crystal structures of bovine odorant-binding protein in complex with odorant molecules.,Vincent F, Ramoni R, Spinelli S, Grolli S, Tegoni M, Cambillau C Eur J Biochem. 2004 Oct;271(19):3832-42. PMID:15373829<ref>PMID:15373829</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1gt5" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
The structure of bovine odorant-binding protein (bOBP) revealed a striking feature of a dimer formed by domain swapping [Tegoni, M., Ramoni, R., Bignetti, E., Spinelli, S. &amp; Cambillau, C. (1996) Nat. Struct. Biol.3, 863-867; Bianchet, M.A., Bains, G., Pelosi, P., Pevsner, J., Snyder, S.H., Monaco, H.L. &amp; Amzel, L.M. (1996) Nat. Struct. Biol.3, 934-939] and the presence of a naturally occuring ligand [Ramoni, R., Vincent, F., Grolli, S., Conti, V., Malosse, C., Boyer, F.D., Nagnan-Le Meillour, P., Spinelli, S., Cambillau, C. &amp; Tegoni, M. (2001) J. Biol. Chem.276, 7150-7155]. These features led us to investigate the binding of odorant molecules with bOBP in solution and in the crystal. The behavior of odorant molecules in bOBP resembles that observed with porcine OBP (pOBP), although the latter is monomeric and devoid of ligand when purified. The odorant molecules presented K(d) values with bOBP in the micromolar range. Most of the X-ray structures revealed that odorant molecules interact with a common set of residues forming the cavity wall and do not exhibit specific interactions. Depending on the ligand and on the monomer (A or B), a single residue--Phe89--presents alternate conformations and might control cross-talking between the subunits. Crystal data on both pOBP and bOBP, in contrast with binding and spectroscopic studies on rat OBP in solution, reveal an absence of significant conformational changes involving protein loops or backbone. Thus, the role of OBP in signal triggering remains unresolved.
*[[Odorant binding protein 3D structures|Odorant binding protein 3D structures]]
 
== References ==
==About this Structure==
<references/>
1GT5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GT5 OCA].
__TOC__
 
</StructureSection>
==Reference==
Crystal structures of bovine odorant-binding protein in complex with odorant molecules., Vincent F, Ramoni R, Spinelli S, Grolli S, Tegoni M, Cambillau C, Eur J Biochem. 2004 Oct;271(19):3832-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15373829 15373829]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cambillau, C.]]
[[Category: Cambillau C]]
[[Category: Conti, V.]]
[[Category: Conti V]]
[[Category: Grolli, S.]]
[[Category: Grolli S]]
[[Category: Ramoni, R.]]
[[Category: Ramoni R]]
[[Category: Spinelli, S.]]
[[Category: Spinelli S]]
[[Category: Tegoni, M.]]
[[Category: Tegoni M]]
[[Category: Vincent, F.]]
[[Category: Vincent F]]
[[Category: BZQ]]
[[Category: bovine odorant binding protein]]
[[Category: lipocalin]]
 
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