1e6e: Difference between revisions

<StructureSection load='1e6e' size='340' side='right'caption='[[1e6e]], [[Resolution|resolution]] 2.30&Aring;' scene=''>

== Structural highlights ==

<table><tr><td colspan='2'>[[1e6e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. The October 2006 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Cytochrome p450''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2006_10 10.2210/rcsb_pdb/mom_2006_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E6E FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e6e OCA], [https://pdbe.org/1e6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e6e RCSB], [https://www.ebi.ac.uk/pdbsum/1e6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e6e ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/ADRO_BOVIN ADRO_BOVIN] Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.

[[Image:Consurf_key_small.gif|200px|right]]

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e6e ConSurf].

== Publication Abstract from PubMed ==

The steroid hydroxylating system of adrenal cortex mitochondria consists of the membrane-attached NADPH-dependent adrenodoxin reductase (AR), the soluble one-electron transport protein adrenodoxin (Adx), and a membrane-integrated cytochrome P450 of the CYP11 family. In the 2.3-A resolution crystal structure of the Adx.AR complex, 580 A(2) of partly polar surface are buried. Main interaction sites are centered around Asp(79), Asp(76), Asp(72), and Asp(39) of Adx and around Arg(211), Arg(240), Arg(244), and Lys(27) of AR, respectively. In particular, the region around Asp(39) defines a new protein interaction site for Adx, similar to those found in plant and bacterial ferredoxins. Additional contacts involve the electron transfer region between the redox centers of AR and Adx and C-terminal residues of Adx. The Adx residues Asp(113) to Arg(115) adopt 3(10)-helical conformation and engage in loose intermolecular contacts within a deep cleft of AR. Complex formation is accompanied by a slight domain rearrangement in AR. The [2Fe-2S] cluster of Adx and the isoalloxazine rings of FAD of AR are 10 A apart suggesting a possible electron transfer route between these redox centers. The AR.Adx complex represents the first structure of a biologically relevant complex between a ferredoxin and its reductase.

Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis.,Muller JJ, Lapko A, Bourenkov G, Ruckpaul K, Heinemann U J Biol Chem. 2001 Jan 26;276(4):2786-9. Epub 2000 Oct 25. PMID:11053423<ref>PMID:11053423</ref>

==See Also==

*[[Adrenodoxin reductase 3D structures|Adrenodoxin reductase 3D structures]]

*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]

== References ==

<references/>

</StructureSection>

[[Category: Large Structures]]

[[Category: RCSB PDB Molecule of the Month]]

[[Category: Bourenkov G]]

[[Category: Heinemann U]]

[[Category: Lapko A]]

[[Category: Mueller JJ]]

[[Category: Ruckpaul K]]