Sulfatase-modifying factor: Difference between revisions
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<StructureSection load='2aij' size=' | <StructureSection load='2aij' size='400' side='right' caption='Glycosylated human sulfatase-modifying factor 1 (cyan) complex with arylsulfatase peptide (magenta), Ca+2 ion (green) and Cl- ion (green) (PDB code [[2aij]]) ' scene='80/801257/Cv/1'> | ||
== Function == | == Function == | ||
'''Sulfatase-modifying factor''' (SUMF) is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing posttranslationally a cysteine residue in its substrate [[Sulfatase]] active site to 3-oxoalanine (C-α-formylglycine) residue<ref>PMID:17446859</ref>. | '''Sulfatase-modifying factor''' (SUMF) or '''formylglycine-generating enzyme''' is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing posttranslationally a cysteine residue in its substrate [[Sulfatase]] active site to 3-oxoalanine (C-α-formylglycine) residue<ref>PMID:17446859</ref>. | ||
== Disease == | == Disease == | ||
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== Structural highlights == | == Structural highlights == | ||
The structure of SUMF1 complex with its substrate sulfatase terminal peptide CTPSR. | <scene name='80/801257/Cv/9'>Glycosylated human sulfatase-modifying factor 1 complex with arylsulfatase peptide, Ca+2 ion and Cl- ion</scene>. The structure of SUMF1 complex with its substrate sulfatase terminal peptide CTPSR. <scene name='80/801257/Cv/10'>SUMF1 active site contains 2 cysteine residues and mutating either of them to serine results in an inactive enzyme</scene>. Cys341 was found to be <scene name='80/801257/Cv/11'>responsible for substrate binding and makes a Cys-Cys bond</scene> to the peptide cysteine residue. The peptide binds at the surface of SUMF1 in an extended conformation <scene name='80/801257/Cv/13'>making numerous interactions with the protein</scene><ref>PMID:16368756</ref>. | ||
*<scene name='80/801257/Cv/14'>Cl coordination site</scene>. Water molecules are shown as red spheres. | |||
*<scene name='80/801257/Cv/15'>1st Ca coordination site</scene>. | |||
*<scene name='80/801257/Cv/16'>2nd Ca coordination site</scene>. | |||
</StructureSection> | </StructureSection> | ||
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
[[2aii]] - hSUMF-1 - human<br /> | [[2aii]], [[1y1e]], [[1y1g]], [[1y1f]], [[1y1j]], [[1y1i]], [[1z70]], [[1y1h]], [[5ssy]], [[5ssz]] - hSUMF-1 - human<br /> | ||
[[2aft]], [[2afy]], [[2hib]], [[2hi8]] - hSUMF-1 (mutant) <br /> | [[2aft]], [[2afy]], [[2hib]], [[2hi8]], [[5ssx]], [[8aru]] - hSUMF-1 (mutant) <br /> | ||
[[2aij]], [[2aik]] - hSUMF-1 (mutant) + peptide<br /> | [[2aij]], [[2aik]] - hSUMF-1 (mutant) + peptide<br /> | ||
[[1y4j]] - hSUMF-2 <br /> | [[1y4j]] - hSUMF-2 <br /> | ||