5o98: Difference between revisions
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==Binary complex of Catharanthus roseus Vitrosamine Synthase with NADP+== | |||
<StructureSection load='5o98' size='340' side='right'caption='[[5o98]], [[Resolution|resolution]] 1.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5o98]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Catharanthus_roseus Catharanthus roseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O98 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o98 OCA], [https://pdbe.org/5o98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o98 RCSB], [https://www.ebi.ac.uk/pdbsum/5o98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o98 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0C5DR25_CATRO A0A0C5DR25_CATRO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Monoterpene indole alkaloids, a large class of plant natural products, derive from the biosynthetic intermediate strictosidine aglycone. Strictosidine aglycone, which can exist as a variety of isomers, can be reduced to form numerous different structures. We have discovered a short chain alcohol dehydrogenase (SDR) from monoterpene indole alkaloid producing plants (Catharanthus roseus and Rauvolfia serpentina) that reduce strictosidine aglycone and produce an alkaloid that does not correspond to any previously reported compound. Here we report the structural characterization of this product, which we have named vitrosamine, as well as the crystal structure of the SDR. This discovery highlights the structural versatility of the strictosidine aglycone biosynthetic intermediate and expands the range of enzymatic reactions that SDRs can catalyze. This discovery further highlights how a sequence-based gene mining discovery approach in plants can reveal cryptic chemistry that would not be uncovered by classical natural product chemistry approaches. | |||
Discovery of a short chain dehydrogenase from Catharanthus roseus that produces a novel monoterpene indole alkaloid.,Stavrinides A, Tatsis E, Dang TT, Caputi L, Stevenson C, Lawson D, Schneider B, O'Connor S Chembiochem. 2018 Feb 9. doi: 10.1002/cbic.201700621. PMID:29424954<ref>PMID:29424954</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5o98" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Catharanthus roseus]] | |||
[[Category: Large Structures]] | |||
[[Category: Caputi L]] | |||
[[Category: Dang TT]] | |||
[[Category: Lawson DM]] | |||
[[Category: O'Connor SE]] | |||
[[Category: Schneider B]] | |||
[[Category: Stavrinides AK]] | |||
[[Category: Stevenson CEM]] | |||
[[Category: Tatsis EC]] | |||
Latest revision as of 12:37, 6 December 2023
Binary complex of Catharanthus roseus Vitrosamine Synthase with NADP+Binary complex of Catharanthus roseus Vitrosamine Synthase with NADP+
Structural highlights
FunctionPublication Abstract from PubMedMonoterpene indole alkaloids, a large class of plant natural products, derive from the biosynthetic intermediate strictosidine aglycone. Strictosidine aglycone, which can exist as a variety of isomers, can be reduced to form numerous different structures. We have discovered a short chain alcohol dehydrogenase (SDR) from monoterpene indole alkaloid producing plants (Catharanthus roseus and Rauvolfia serpentina) that reduce strictosidine aglycone and produce an alkaloid that does not correspond to any previously reported compound. Here we report the structural characterization of this product, which we have named vitrosamine, as well as the crystal structure of the SDR. This discovery highlights the structural versatility of the strictosidine aglycone biosynthetic intermediate and expands the range of enzymatic reactions that SDRs can catalyze. This discovery further highlights how a sequence-based gene mining discovery approach in plants can reveal cryptic chemistry that would not be uncovered by classical natural product chemistry approaches. Discovery of a short chain dehydrogenase from Catharanthus roseus that produces a novel monoterpene indole alkaloid.,Stavrinides A, Tatsis E, Dang TT, Caputi L, Stevenson C, Lawson D, Schneider B, O'Connor S Chembiochem. 2018 Feb 9. doi: 10.1002/cbic.201700621. PMID:29424954[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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