1zxa: Difference between revisions

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-[[Image:1zxa.gif|left|200px]]<br />
-<applet load="1zxa" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1zxa" />
-'''Solution Structure of the Coiled-Coil Domain of cGMP-dependent Protein Kinase Ia'''<br />
-==Overview==
+==Solution Structure of the Coiled-Coil Domain of cGMP-dependent Protein Kinase Ia==
-Coiled-coil motifs play essential roles in protein assembly and molecular, recognition, and are therefore the targets of many ongoing structural and, functional studies. However, owing to the dynamic nature of many of the, smaller coiled-coil domains, crystallization for X-ray studies is very, challenging. Determination of elongated structures using standard NMR, approaches is inefficient and usually yields low-resolution structures due, to accumulation of small errors over long distances. Here we describe a, solution NMR approach based on residual dipolar couplings (RDCs) for rapid, and accurate structure determination of coiled-coil dimers. Using this, approach, we were able to determine the high-resolution structure of the, coiled-coil domain of cGMP-dependent protein kinase Ialpha, a protein of, previously unknown structure that is critical for physiological relaxation, of vascular smooth muscle. This approach can be extended to solve, coiled-coil structures with higher order assemblies.
+<StructureSection load='1zxa' size='340' side='right'caption='[[1zxa]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1zxa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZXA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZXA FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zxa OCA], [https://pdbe.org/1zxa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zxa RCSB], [https://www.ebi.ac.uk/pdbsum/1zxa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zxa ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/KGP1_HUMAN KGP1_HUMAN] Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.<ref>PMID:8182057</ref> <ref>PMID:10567269</ref> <ref>PMID:11162591</ref> <ref>PMID:12082086</ref> <ref>PMID:11723116</ref> <ref>PMID:14608379</ref> <ref>PMID:15194681</ref> <ref>PMID:16990611</ref> <ref>PMID:21402151</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Coiled-coil motifs play essential roles in protein assembly and molecular recognition, and are therefore the targets of many ongoing structural and functional studies. However, owing to the dynamic nature of many of the smaller coiled-coil domains, crystallization for X-ray studies is very challenging. Determination of elongated structures using standard NMR approaches is inefficient and usually yields low-resolution structures due to accumulation of small errors over long distances. Here we describe a solution NMR approach based on residual dipolar couplings (RDCs) for rapid and accurate structure determination of coiled-coil dimers. Using this approach, we were able to determine the high-resolution structure of the coiled-coil domain of cGMP-dependent protein kinase Ialpha, a protein of previously unknown structure that is critical for physiological relaxation of vascular smooth muscle. This approach can be extended to solve coiled-coil structures with higher order assemblies.
-==About this Structure==
+Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha.,Schnell JR, Zhou GP, Zweckstetter M, Rigby AC, Chou JJ Protein Sci. 2005 Sep;14(9):2421-8. PMID:16131665<ref>PMID:16131665</ref>
-ZXA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZXA OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha., Schnell JR, Zhou GP, Zweckstetter M, Rigby AC, Chou JJ, Protein Sci. 2005 Sep;14(9):2421-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16131665 16131665]
+</div>
+<div class="pdbe-citations 1zxa" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Non-specific serine/threonine protein kinase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Chou JJ]]
-[[Category: Chou, J.J.]]
+[[Category: Rigby AC]]
-[[Category: Rigby, A.C.]]
+[[Category: Schnell JR]]
-[[Category: Schnell, J.R.]]
+[[Category: Zhou GP]]
-[[Category: Zhou, G.P.]]
+[[Category: Zweckstetter M]]
-[[Category: Zweckstetter, M.]]
-[[Category: parallel coiled coil dimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:42:45 2007''