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[[Image:1tan.gif|left|200px]]


{{Structure
==TANDEM DNA, NMR, MINIMIZED AVERAGE STRUCTURE==
|PDB= 1tan |SIZE=350|CAPTION= <scene name='initialview01'>1tan</scene>
<StructureSection load='1tan' size='340' side='right'caption='[[1tan]]' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1tan]] is a 3 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TAN FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tan OCA], [https://pdbe.org/1tan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tan RCSB], [https://www.ebi.ac.uk/pdbsum/1tan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tan ProSAT]</span></td></tr>
}}
</table>
 
<div style="background-color:#fffaf0;">
'''TANDEM DNA, NMR, MINIMIZED AVERAGE STRUCTURE'''
== Publication Abstract from PubMed ==
 
 
==Overview==
The solution structure of an estrone (Es)-tethered tandem DNA duplex consisting of two Es-tethered tetranucleotides and a target octameric DNA sequence is reported. The structure of this Es-tethered tandem duplex has been compared with a corresponding natural tandem duplex without estrones. The Tm of the 3'-Es-tethered tetranucleotide part of the tandem duplex increases by 5 degrees C, whereas the Tm of the 5'-Es-tethered tetranucleotide part increases by 7 degrees C, compared with the corresponding natural counterpart. The NMR structures of both the Es-tethered tandem duplex and the natural counterpart have been based on 24 experimental NMR constraints per residue. Despite the fact that there is considerable distortion at the junction of two Es-tethered tetranucleotides in the major groove of the Es-tethered DNA duplex compared to the natural counterpart, both duplexes do take up B-type DNA structures. It is likely that the spatial proximity of two Es residues, and the resulting hydrophobic interaction between them might be responsible for the increase of the thermal stability of the Es-tethered tandem duplex in comparison with the natural counterpart.
The solution structure of an estrone (Es)-tethered tandem DNA duplex consisting of two Es-tethered tetranucleotides and a target octameric DNA sequence is reported. The structure of this Es-tethered tandem duplex has been compared with a corresponding natural tandem duplex without estrones. The Tm of the 3'-Es-tethered tetranucleotide part of the tandem duplex increases by 5 degrees C, whereas the Tm of the 5'-Es-tethered tetranucleotide part increases by 7 degrees C, compared with the corresponding natural counterpart. The NMR structures of both the Es-tethered tandem duplex and the natural counterpart have been based on 24 experimental NMR constraints per residue. Despite the fact that there is considerable distortion at the junction of two Es-tethered tetranucleotides in the major groove of the Es-tethered DNA duplex compared to the natural counterpart, both duplexes do take up B-type DNA structures. It is likely that the spatial proximity of two Es residues, and the resulting hydrophobic interaction between them might be responsible for the increase of the thermal stability of the Es-tethered tandem duplex in comparison with the natural counterpart.


==About this Structure==
The NMR structure of estrone (Es)-tethered tandem DNA duplex: [d(5'pCAGCp3')-Es] + [Es-d(5'pTCCA3')]: d(5'pTGGAGCTG3').,Denisov AY, Sandstrom A, Maltseva TV, Pyshnyi DV, Ivanova EM, Zarytova VF, Chattopadhyaya J J Biomol Struct Dyn. 1997 Dec;15(3):499-516. PMID:9439997<ref>PMID:9439997</ref>
1TAN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAN OCA].
 
==Reference==
The NMR structure of estrone (Es)-tethered tandem DNA duplex: [d(5'pCAGCp3')-Es] + [Es-d(5'pTCCA3')]: d(5'pTGGAGCTG3')., Denisov AY, Sandstrom A, Maltseva TV, Pyshnyi DV, Ivanova EM, Zarytova VF, Chattopadhyaya J, J Biomol Struct Dyn. 1997 Dec;15(3):499-516. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9439997 9439997]
[[Category: Protein complex]]
[[Category: Chattopadhyaya, J.]]
[[Category: Denisov, A.]]
[[Category: Ivanova, E.]]
[[Category: Maltseva, T.]]
[[Category: Pyshnyi, D.]]
[[Category: Sandstrom, A.]]
[[Category: Zarytova, V.]]
[[Category: deoxyribonucleic acid]]
[[Category: dna]]
[[Category: nmr]]
[[Category: tandem]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:16:03 2008''
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1tan" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Chattopadhyaya J]]
[[Category: Denisov A]]
[[Category: Ivanova E]]
[[Category: Maltseva T]]
[[Category: Pyshnyi D]]
[[Category: Sandstrom A]]
[[Category: Zarytova V]]

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