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== | ==TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES== | ||
<StructureSection load='1qes' size='340' side='right'caption='[[1qes]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1qes]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QES FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qes OCA], [https://pdbe.org/1qes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qes RCSB], [https://www.ebi.ac.uk/pdbsum/1qes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qes ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences. | The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences. | ||
Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2.,McDowell JA, He L, Chen X, Turner DH Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950<ref>PMID:9201950</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1qes" style="background-color:#fffaf0;"></div> | ||
[[Category: Chen | == References == | ||
[[Category: He | <references/> | ||
[[Category: Mcdowell | __TOC__ | ||
[[Category: Turner | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Chen X]] | |||
[[Category: He L]] | |||
[[Category: Mcdowell JA]] | |||
[[Category: Turner DH]] | |||
Latest revision as of 12:19, 6 December 2023
TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURESTANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES
Structural highlights
Publication Abstract from PubMedThe symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences. Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2.,McDowell JA, He L, Chen X, Turner DH Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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