1n1k: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:1n1k.png|left|200px]]
-<!--
+==NMR Structure for d(CCGCGG)2==
-The line below this paragraph, containing "STRUCTURE_1n1k", creates the "Structure Box" on the page.
+<StructureSection load='1n1k' size='340' side='right'caption='[[1n1k]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1n1k]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N1K FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n1k OCA], [https://pdbe.org/1n1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n1k RCSB], [https://www.ebi.ac.uk/pdbsum/1n1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n1k ProSAT]</span></td></tr>
-{{STRUCTURE_1n1k|  PDB=1n1k  |  SCENE=  }}
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Long repeated stretches of d(CCG) and tri-nucleotide are crucial mutations that cause hereditary forms of mental retardation (fragile X-syndrome). Moreover, the alternating (CG) di-nucleotide is one of the candidates for Z-DNA conformation. Solution NMR structure of d(CCGCGG)(2) has been solved and is discussed. The determined NMR solution structure is a distorted highly bent B-DNA conformation with increased flexibility in both terminal residues. This conformation differs significantly from the Z-DNA tetramer structure reported for the same hexamer in the crystal state at similar ionic strength by Malinina and co-workers. Crystal structure of d(CCGCGG)(2) at high salt concentration includes a central alternating tetramer in Z-DNA conformation, while the initial cytosine swings out and forms a Watson-Crick base-pair with the terminal guanine of a symmetry-related molecule. In solution, NMR data for sugar ring puckering combined with restrained molecular dynamics simulations starting from a Z-DNA form show that terminal furanose residues could adopt the conformation required for aromatic bases swinging out. Therefore, tetramer formation could be considered possible once the hexanucleotide had previously adopted the Z-DNA form. This work gives some insight into correlations between anomalous crystal structures and their accessibility in the solution state.
-===NMR Structure for d(CCGCGG)2===
+NMR study of hexanucleotide d(CCGCGG)2 containing two triplet repeats of fragile X syndrome.,Monleon D, Esteve V, Celda B Biochem Biophys Res Commun. 2003 Mar 28;303(1):81-90. PMID:12646170<ref>PMID:12646170</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_12646170}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1n1k" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 12646170 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_12646170}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1K OCA].
+[[Category: Celda B]]
+[[Category: Monleon D]]
-==Reference==
-NMR study of hexanucleotide d(CCGCGG)2 containing two triplet repeats of fragile X syndrome., Monleon D, Esteve V, Celda B, Biochem Biophys Res Commun. 2003 Mar 28;303(1):81-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12646170 12646170]
-[[Category: Celda, B.]]
-[[Category: Monleon, D.]]
-[[Category: Ccgcgg]]
-[[Category: Nmr]]
-[[Category: Recombination-like]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:17:41 2008''