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==Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine== | ==Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine== | ||
<StructureSection load='1lpw' size='340' side='right' caption='[[1lpw | <StructureSection load='1lpw' size='340' side='right'caption='[[1lpw]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1lpw]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1lpw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LPW FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lpw OCA], [https://pdbe.org/1lpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lpw RCSB], [https://www.ebi.ac.uk/pdbsum/1lpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lpw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 1lpw" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Greenbaum | [[Category: Greenbaum NL]] | ||
[[Category: Newby | [[Category: Newby MI]] | ||
Latest revision as of 21:48, 29 November 2023
Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridineSolution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine
Structural highlights
Publication Abstract from PubMedPairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing. Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.,Newby MI, Greenbaum NL Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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