1kr8: Difference between revisions
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==Refinement of d(GCGAAGC) Hairpin Structure Using One-and Two-Bonds Residual Dipolar Couplings== | |||
<StructureSection load='1kr8' size='340' side='right'caption='[[1kr8]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1kr8]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KR8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kr8 OCA], [https://pdbe.org/1kr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kr8 RCSB], [https://www.ebi.ac.uk/pdbsum/1kr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kr8 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structure of the 13C,15N-labeled d(GCGAAGC) hairpin, as determined by NMR spectroscopy and refined using molecular dynamics with NOE-derived distances, torsion angles, and residual dipolar couplings (RDCs), is presented. Although the studied molecule is of small size, it is demonstrated that the incorporation of diminutive RDCs can significantly improve local structure determination of regions undefined by the conventional restraints. Very good correlation between the experimental and back-calculated small one- and two-bond 1H-13C, 1H-15N, 13C-13C and 13C-15N coupling constants has been attained. The final structures clearly show typical features of the miniloop architecture. The structure is discussed in context of the extraordinary stability of the d(GCGAAGC) hairpin, which originates from a complex interplay between the aromatic base stacking and hydrogen bonding interactions. | |||
Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings.,Padrta P, Stefl R, Kralik L, Zidek L, Sklenar V J Biomol NMR. 2002 Sep;24(1):1-14. PMID:12449414<ref>PMID:12449414</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1kr8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
< | </StructureSection> | ||
[[Category: Padrta | [[Category: Large Structures]] | ||
[[Category: Sklenar | [[Category: Padrta P]] | ||
[[Category: Stefl | [[Category: Sklenar V]] | ||
[[Category: Zidek | [[Category: Stefl R]] | ||
[[Category: Zidek L]] | |||
Latest revision as of 21:46, 29 November 2023
Refinement of d(GCGAAGC) Hairpin Structure Using One-and Two-Bonds Residual Dipolar CouplingsRefinement of d(GCGAAGC) Hairpin Structure Using One-and Two-Bonds Residual Dipolar Couplings
Structural highlights
Publication Abstract from PubMedThe structure of the 13C,15N-labeled d(GCGAAGC) hairpin, as determined by NMR spectroscopy and refined using molecular dynamics with NOE-derived distances, torsion angles, and residual dipolar couplings (RDCs), is presented. Although the studied molecule is of small size, it is demonstrated that the incorporation of diminutive RDCs can significantly improve local structure determination of regions undefined by the conventional restraints. Very good correlation between the experimental and back-calculated small one- and two-bond 1H-13C, 1H-15N, 13C-13C and 13C-15N coupling constants has been attained. The final structures clearly show typical features of the miniloop architecture. The structure is discussed in context of the extraordinary stability of the d(GCGAAGC) hairpin, which originates from a complex interplay between the aromatic base stacking and hydrogen bonding interactions. Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings.,Padrta P, Stefl R, Kralik L, Zidek L, Sklenar V J Biomol NMR. 2002 Sep;24(1):1-14. PMID:12449414[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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