1fsv: Difference between revisions

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-{{Seed}}
-[[Image:1fsv.png|left|200px]]
-<!--
+==FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, MINIMIZED AVERAGE STRUCTURE==
-The line below this paragraph, containing "STRUCTURE_1fsv", creates the "Structure Box" on the page.
+<StructureSection load='1fsv' size='340' side='right'caption='[[1fsv]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1fsv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FSV FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fsv OCA], [https://pdbe.org/1fsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fsv RCSB], [https://www.ebi.ac.uk/pdbsum/1fsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fsv ProSAT]</span></td></tr>
-{{STRUCTURE_1fsv|  PDB=1fsv  |  SCENE=  }}
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The first fully automated design and experimental validation of a novel sequence for an entire protein is described. A computational design algorithm based on physical chemical potential functions and stereochemical constraints was used to screen a combinatorial library of 1.9 x 10(27) possible amino acid sequences for compatibility with the design target, a betabetaalpha protein motif based on the polypeptide backbone structure of a zinc finger domain. A BLAST search shows that the designed sequence, full sequence design 1 (FSD-1), has very low identity to any known protein sequence. The solution structure of FSD-1 was solved by nuclear magnetic resonance spectroscopy and indicates that FSD-1 forms a compact well-ordered structure, which is in excellent agreement with the design target structure. This result demonstrates that computational methods can perform the immense combinatorial search required for protein design, and it suggests that an unbiased and quantitative algorithm can be used in various structural contexts.
-===FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, MINIMIZED AVERAGE STRUCTURE===
+De novo protein design: fully automated sequence selection.,Dahiyat BI, Mayo SL Science. 1997 Oct 3;278(5335):82-7. PMID:9311930<ref>PMID:9311930</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_9311930}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1fsv" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 9311930 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9311930}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FSV OCA].
+[[Category: Synthetic construct]]
+[[Category: Dahiyat BI]]
-==Reference==
+[[Category: Mayo SL]]
-De novo protein design: fully automated sequence selection., Dahiyat BI, Mayo SL, Science. 1997 Oct 3;278(5335):82-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9311930 9311930]
-[[Category: Dahiyat, B I.]]
-[[Category: Mayo, S L.]]
-[[Category: Beta beta alpha motif]]
-[[Category: Computational design]]
-[[Category: Novel sequence]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 03:53:46 2008''