1fsv: Difference between revisions

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-[[Image:1fsv.png|left|200px]]
-{{STRUCTURE_1fsv|  PDB=1fsv  |  SCENE=  }}
+==FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, MINIMIZED AVERAGE STRUCTURE==
+<StructureSection load='1fsv' size='340' side='right'caption='[[1fsv]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1fsv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FSV FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fsv OCA], [https://pdbe.org/1fsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fsv RCSB], [https://www.ebi.ac.uk/pdbsum/1fsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fsv ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The first fully automated design and experimental validation of a novel sequence for an entire protein is described. A computational design algorithm based on physical chemical potential functions and stereochemical constraints was used to screen a combinatorial library of 1.9 x 10(27) possible amino acid sequences for compatibility with the design target, a betabetaalpha protein motif based on the polypeptide backbone structure of a zinc finger domain. A BLAST search shows that the designed sequence, full sequence design 1 (FSD-1), has very low identity to any known protein sequence. The solution structure of FSD-1 was solved by nuclear magnetic resonance spectroscopy and indicates that FSD-1 forms a compact well-ordered structure, which is in excellent agreement with the design target structure. This result demonstrates that computational methods can perform the immense combinatorial search required for protein design, and it suggests that an unbiased and quantitative algorithm can be used in various structural contexts.
-===FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, MINIMIZED AVERAGE STRUCTURE===
+De novo protein design: fully automated sequence selection.,Dahiyat BI, Mayo SL Science. 1997 Oct 3;278(5335):82-7. PMID:9311930<ref>PMID:9311930</ref>
-{{ABSTRACT_PUBMED_9311930}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1fsv" style="background-color:#fffaf0;"></div>
-[[1fsv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FSV OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:009311930</ref><references group="xtra"/>
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Synthetic construct]]
-[[Category: Dahiyat, B I.]]
+[[Category: Dahiyat BI]]
-[[Category: Mayo, S L.]]
+[[Category: Mayo SL]]
-[[Category: Beta beta alpha motif]]
-[[Category: Computational design]]
-[[Category: Novel sequence]]