1fsd: Difference between revisions
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==FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, 41 STRUCTURES== | ==FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, 41 STRUCTURES== | ||
<StructureSection load='1fsd' size='340' side='right' caption='[[1fsd | <StructureSection load='1fsd' size='340' side='right'caption='[[1fsd]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1fsd]] is a 1 chain structure. The October 2005 RCSB PDB [ | <table><tr><td colspan='2'>[[1fsd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. The October 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Designer Proteins'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_10 10.2210/rcsb_pdb/mom_2005_10]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FSD FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fsd OCA], [https://pdbe.org/1fsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fsd RCSB], [https://www.ebi.ac.uk/pdbsum/1fsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fsd ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 20: | Line 21: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Designer Proteins]] | [[Category: Designer Proteins]] | ||
[[Category: Large Structures]] | |||
[[Category: RCSB PDB Molecule of the Month]] | [[Category: RCSB PDB Molecule of the Month]] | ||
[[Category: | [[Category: Synthetic construct]] | ||
[[Category: | [[Category: Dahiyat BI]] | ||
[[Category: | [[Category: Mayo SL]] | ||
Latest revision as of 21:36, 29 November 2023
FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, 41 STRUCTURESFULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, 41 STRUCTURES
Structural highlights
Publication Abstract from PubMedThe first fully automated design and experimental validation of a novel sequence for an entire protein is described. A computational design algorithm based on physical chemical potential functions and stereochemical constraints was used to screen a combinatorial library of 1.9 x 10(27) possible amino acid sequences for compatibility with the design target, a betabetaalpha protein motif based on the polypeptide backbone structure of a zinc finger domain. A BLAST search shows that the designed sequence, full sequence design 1 (FSD-1), has very low identity to any known protein sequence. The solution structure of FSD-1 was solved by nuclear magnetic resonance spectroscopy and indicates that FSD-1 forms a compact well-ordered structure, which is in excellent agreement with the design target structure. This result demonstrates that computational methods can perform the immense combinatorial search required for protein design, and it suggests that an unbiased and quantitative algorithm can be used in various structural contexts. De novo protein design: fully automated sequence selection.,Dahiyat BI, Mayo SL Science. 1997 Oct 3;278(5335):82-7. PMID:9311930[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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