7eyt: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 7eyt is ON HOLD
+==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG==
+<StructureSection load='7eyt' size='340' side='right'caption='[[7eyt]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7eyt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_sp. Aspergillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0I3:Andilesin+C'>0I3</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=OGA:N-OXALYLGLYCINE'>OGA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyt OCA], [https://pdbe.org/7eyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyt RCSB], [https://www.ebi.ac.uk/pdbsum/7eyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyt ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A6J4CX17_9EURO A0A6J4CX17_9EURO]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Non-heme iron and alpha-ketoglutarate-dependent (Fe/alphaKG) oxygenases catalyze various oxidative biotransformations. Due to their catalytic flexibility and high efficiency, Fe/alphaKG oxygenases have attracted keen attention for their application as biocatalysts. Here, we report the biochemical and structural characterizations of the unusually promiscuous and catalytically versatile Fe/alphaKG oxygenase SptF, involved in the biosynthesis of fungal meroterpenoid emervaridones. The in vitro analysis revealed that SptF catalyzes several continuous oxidation reactions, including hydroxylation, desaturation, epoxidation, and skeletal rearrangement. SptF exhibits extremely broad substrate specificity toward various meroterpenoids, and efficiently produced unique cyclopropane-ring-fused 5/3/5/5/6/6 and 5/3/6/6/6 scaffolds from terretonins. Moreover, SptF also hydroxylates steroids, including androsterone, testosterone, and progesterone, with different regiospecificities. Crystallographic and structure-based mutagenesis studies of SptF revealed the molecular basis of the enzyme reactions, and suggested that the malleability of the loop region contributes to the remarkable substrate promiscuity. SptF exhibits great potential as a promising biocatalyst for oxidation reactions.
-Authors: Tao, H., Mori, T., Abe, I.
+Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/alpha-ketoglutarate-dependent oxygenase SptF.,Tao H, Mori T, Chen H, Lyu S, Nonoyama A, Lee S, Abe I Nat Commun. 2022 Jan 10;13(1):95. doi: 10.1038/s41467-021-27636-3. PMID:35013177<ref>PMID:35013177</ref>
-Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF with andilesin C and NOG
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Abe, I]]
+<div class="pdbe-citations 7eyt" style="background-color:#fffaf0;"></div>
-[[Category: Mori, T]]
-[[Category: Tao, H]]
+==See Also==
+*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Aspergillus sp]]
+[[Category: Large Structures]]
+[[Category: Abe I]]
+[[Category: Mori T]]
+[[Category: Tao H]]